LOCUS SIU02238.1 469 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CYSTEINYL-
TRNA SYNTHETASE 1 CYSS1 (CYSTEINE--TRNA LIGASE 1) (CYSRS
1) (CYSTEINE TRANSLASE) protein.
ACCESSION LT708304-3652
PROTEIN_ID SIU02238.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="cysS1"
/locus_tag="BQ2027_MB3611C"
/note="Mb3611c, cysS1, len: 469 aa. Equivalent to Rv3580c,
len: 469 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 469 aa overlap). Probable cysS1,
cysteinyl-tRNA synthetase (EC 6.1.1.16), equivalent to
P57990|SYC1_MYCLE|CYSS1|CYSS|ML0323 CYSTEINYL-TRNA
SYNTHETASE 1 from Mycobacterium leprae (473 aa) FASTA
scores: opt: 2825, E(): 3.4e-172, (86.5% identity in 467
aa overlap). Also similar to many e.g. Q9L0Q6|SCD8A.08
from Streptomyces coelicolor (613 aa), FASTA scores: opt:
1834, E(): 4.7e-109, (57.5% identity in 461 aa overlap);
Q9I2U7|CYSS|PA1795 from Pseudomonas aeruginosa (460 aa)
FASTA scores: opt: 1197, E(): 1.2e-68, (41.65% identity in
468 aa overlap); P21888|SYC_ECOLI P21888|CYSS|B0526 from
Escherichia coli strain K12 (461 aa), FASTA scores: opt:
1189, E(): 4e-68, (43.0% identity in 463 aa overlap); etc.
BELONGS TO CLASS-I AMINOACYL-TRNA SYNTHETASE FAMILY.
STRONGLY SIMILAR TO METHIONYL-TRNA SYNTHETASE. Protein
product from Mb3611c detected using shotgun mass
spectrometry and SWATH mass spectrometry. Mb3611c found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing (TPM > 20)."
/db_xref="GOA:P0A635"
/db_xref="InterPro:IPR009080"
/db_xref="InterPro:IPR014729"
/db_xref="InterPro:IPR015273"
/db_xref="InterPro:IPR015803"
/db_xref="InterPro:IPR024909"
/db_xref="InterPro:IPR032678"
/db_xref="UniProtKB/Swiss-Prot:P0A635"
BEGIN
1 MTDRARLRLH DTAAGVVRDF VPLRPGHVSI YLCGATVQGL PHIGHVRSGV AFDILRRWLL
61 ARGYDVAFIR NVTDIEDKIL AKAAAAGRPW WEWAATHERA FTAAYDALDV LPPSAEPRAT
121 GHITQMIEMI ERLIQAGHAY TGGGDVYFDV LSYPEYGQLS GHKIDDVHQG EGVAAGKRDQ
181 RDFTLWKGEK PGEPSWPTPW GRGRPGWHLE CSAMARSYLG PEFDIHCGGM DLVFPHHENE
241 IAQSRAAGDG FARYWLHNGW VTMGGEKMSK SLGNVLSMPA MLQRVRPAEL RYYLGSAHYR
301 SMLEFSETAM QDAVKAYVGL EDFLHRVRTR VGAVCPGDPT PRFAEALDDD LSVPIALAEI
361 HHVRAEGNRA LDAGDHDGAL RSASAIRAMM GILGCDPLDQ RWESRDETSA ALAAVDVLVQ
421 AELQNREKAR EQRNWALADE IRGRLKRAGI EVTDTADGPQ WSLLGGDTK
//