LOCUS SIU02202.1 433 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CYTOCHROME P450 125 CYP125 protein. ACCESSION LT708304-3616 PROTEIN_ID SIU02202.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cyp125" /locus_tag="BQ2027_MB3575C" /note="Mb3575c, cyp125, len: 433 aa. Equivalent to Rv3545c, len: 433 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 433 aa overlap). Probable cyp125, cytochrome P-450 (EC 1.14.-.-), similar to others e.g. Q59723|LINC|CYP111 from Pseudomonas incognita (406 aa), FASTA scores: opt: 831, E(): 8e-45, (34.75% identity in 406 aa overlap); Q9X8Q3|CYP107P1|SCH10.14c from Streptomyces coelicolor (411 aa), FASTA scores: opt: 694, E(): 3.3e-36, (32.35% identity in 417 aa overlap); Q9L465|CYP162A1|NIKQ from Streptomyces tendae (396 aa) FASTA scores: opt: 664, E(): 2.5e-34, (34.15% identity in 413 aa overlap); O08469|CPXY_BACSU|CYPA|CYP107J1 from Bacillus subtilis (410 aa), FASTA scores: opt: 579, E(): 5.6e-29, (30.05% identity in 366 aa overlap); etc. Also similar to other from Mycobacterium tuberculosis e.g. Q50696|CYP124|Rv2266|MT2328|MTCY339.44c (428 aa) FASTA scores: opt: 1040, E(): 6.1e-58, (40.75% identity in 432 aa overlap). BELONGS TO THE CYTOCHROME P450 FAMILY. Protein product from Mb3575c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3575c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P63710" /db_xref="InterPro:IPR001128" /db_xref="InterPro:IPR002397" /db_xref="InterPro:IPR036396" /db_xref="UniProtKB/Swiss-Prot:P63710" BEGIN 1 MSWNHQSVEI AVRRTTVPSP NLPPGFDFTD PAIYAERLPV AEFAELRSAA PIWWNGQDPG 61 KGGGFHDGGF WAITKLNDVK EISRHSDVFS SYENGVIPRF KNDIAREDIE VQRFVMLNMD 121 APHHTRLRKI ISRGFTPRAV GRLHDELQER AQKIAAEAAA AGSGDFVEQV SCELPLQAIA 181 GLLGVPQEDR GKLFHWSNEM TGNEDPEYAH IDPKASSAEL IGYAMKMAEE KAKNPADDIV 241 TQLIQADIDG EKLSDDEFGF FVVMLAVAGN ETTRNSITQG MMAFAEHPDQ WELYKKVRPE 301 TAADEIVRWA TPVTAFQRTA LRDYELSGVQ IKKGQRVVMF YRSANFDEEV FQDPFTFNIL 361 RNPNPHVGFG GTGAHYCIGA NLARMTINLI FNAVADHMPD LKPISAPERL RSGWLNGIKH 421 WQVDYTGRCP VAH //