LOCUS       SIU02193.1               261 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            HYDRATASE protein.
ACCESSION   LT708304-3607
PROTEIN_ID  SIU02193.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="hsae"
                     /locus_tag="BQ2027_MB3566C"
                     /note="Mb3566c, -, len: 261 aa. Equivalent to Rv3536c,
                     len: 261 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 261 aa overlap). Probable hydratase,
                     2-oxo-hepta-3-ene-1,7-dioate hydratase (EC 4.2.1.-) or
                     2-keto-4-pentenoate hydratase (EC 4.2.1.-). Indeed, highly
                     similar to many 2-oxo-hepta-3-ene-1,7-dioate hydratases
                     e.g. Q9CKS2|HPAH|PM1534 from Pasteurella multocida (267
                     aa) FASTA scores: opt: 743, E(): 1.5e-39, (45.5% identity
                     in 266 aa overlap) Q9RZ31|DRA0122 from Deinococcus
                     radiodurans (268 aa), FASTA scores: opt: 709, E(): 2e-37,
                     (45.5% identity in 266 aa overlap); Q9HWQ4|HPCG|PA4127
                     from Pseudomonas aeruginosa (267 aa), FASTA scores: opt:
                     703, E(): 4.8e-37, (45.1% identity in 266 aa overlap);
                     Q46982|HPAH|HPCG from Escherichia colis strain ATCC 11105
                     (267 aa), FASTA scores: opt: 679, E(): 1.6e-35, (41.35%
                     identity in 266 aa overlap); etc. But also highly similar
                     to many 2-keto-4-pentenoate hydratases
                     (2-hydroxypentadienoic acidhydratases) e.g. Q9LAF7|PHED
                     from Bacillus thermoglucosidasius (258 aa), FASTA scores:
                     opt: 698, E(): 9.7e-37, (42.45% identity in 252 aa
                     overlap); Q52442|BPHH from Pseudomonas sp (260 aa) FASTA
                     scores: opt: 675, E(): 2.7e-35, (41.4% identity in 251 aa
                     overlap); P77608|MHPD_ECOLI|B0350 from Escherichia coli
                     strain K12 (269 aa), FASTA scores: opt: 674, E(): 3.2e-35,
                     (42.75% identity in 255 aa overlap); Q52038|BPHX1 from
                     Pseudomonas pseudoalcaligenes (260 aa), FASTA scores: opt:
                     663, E(): 1.5e-34, (40.6% identity in 251 aa overlap);
                     etc. Protein product from Mb3566c detected using shotgun
                     mass spectrometry and SWATH mass spectrometry. Mb3566c
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y4E3"
                     /db_xref="InterPro:IPR011234"
                     /db_xref="InterPro:IPR036663"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y4E3"
BEGIN
        1 MLRDATRDEL AADLAQAERS RDPIGQLTAA HPEIDVVDAY EIQLINIRQR VAEGARVVGH
       61 KVGLSSPIMQ QMMGVDEPDY GHLLDDMQVF EDTPVQASRY LSPRVEVEVG FILAADLPGA
      121 GCTEDDVLAA TEALVPAIEL IDTRIKDWQI KICDTIADNA SAAGFVLGAA RVPPADLDVR
      181 AIDAKLTRNG EVVAEGRSDA VLGNPATAVA WLAGKVESFG VRLRKGDIVL PGSCTFAVEA
      241 RAGDEFVADF TGLGLVRLSF E
//