LOCUS SIU02193.1 261 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE HYDRATASE protein. ACCESSION LT708304-3607 PROTEIN_ID SIU02193.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="hsae" /locus_tag="BQ2027_MB3566C" /note="Mb3566c, -, len: 261 aa. Equivalent to Rv3536c, len: 261 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 261 aa overlap). Probable hydratase, 2-oxo-hepta-3-ene-1,7-dioate hydratase (EC 4.2.1.-) or 2-keto-4-pentenoate hydratase (EC 4.2.1.-). Indeed, highly similar to many 2-oxo-hepta-3-ene-1,7-dioate hydratases e.g. Q9CKS2|HPAH|PM1534 from Pasteurella multocida (267 aa) FASTA scores: opt: 743, E(): 1.5e-39, (45.5% identity in 266 aa overlap) Q9RZ31|DRA0122 from Deinococcus radiodurans (268 aa), FASTA scores: opt: 709, E(): 2e-37, (45.5% identity in 266 aa overlap); Q9HWQ4|HPCG|PA4127 from Pseudomonas aeruginosa (267 aa), FASTA scores: opt: 703, E(): 4.8e-37, (45.1% identity in 266 aa overlap); Q46982|HPAH|HPCG from Escherichia colis strain ATCC 11105 (267 aa), FASTA scores: opt: 679, E(): 1.6e-35, (41.35% identity in 266 aa overlap); etc. But also highly similar to many 2-keto-4-pentenoate hydratases (2-hydroxypentadienoic acidhydratases) e.g. Q9LAF7|PHED from Bacillus thermoglucosidasius (258 aa), FASTA scores: opt: 698, E(): 9.7e-37, (42.45% identity in 252 aa overlap); Q52442|BPHH from Pseudomonas sp (260 aa) FASTA scores: opt: 675, E(): 2.7e-35, (41.4% identity in 251 aa overlap); P77608|MHPD_ECOLI|B0350 from Escherichia coli strain K12 (269 aa), FASTA scores: opt: 674, E(): 3.2e-35, (42.75% identity in 255 aa overlap); Q52038|BPHX1 from Pseudomonas pseudoalcaligenes (260 aa), FASTA scores: opt: 663, E(): 1.5e-34, (40.6% identity in 251 aa overlap); etc. Protein product from Mb3566c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3566c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y4E3" /db_xref="InterPro:IPR011234" /db_xref="InterPro:IPR036663" /db_xref="UniProtKB/TrEMBL:A0A1R3Y4E3" BEGIN 1 MLRDATRDEL AADLAQAERS RDPIGQLTAA HPEIDVVDAY EIQLINIRQR VAEGARVVGH 61 KVGLSSPIMQ QMMGVDEPDY GHLLDDMQVF EDTPVQASRY LSPRVEVEVG FILAADLPGA 121 GCTEDDVLAA TEALVPAIEL IDTRIKDWQI KICDTIADNA SAAGFVLGAA RVPPADLDVR 181 AIDAKLTRNG EVVAEGRSDA VLGNPATAVA WLAGKVESFG VRLRKGDIVL PGSCTFAVEA 241 RAGDEFVADF TGLGLVRLSF E //