LOCUS       SIU02174.1               193 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            CYTOCHROME P450 MONOOXYGENASE 142 CYP142B [SECOND PART]
            protein.
ACCESSION   LT708304-3588
PROTEIN_ID  SIU02174.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cyp142b"
                     /locus_tag="BQ2027_MB3547C"
                     /note="Mb3547c, cyp142b, len: 193 aa. Equivalent to 3' end
                     of Rv3518c, len: 398 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (100.0% identity in 193 aa overlap).
                     Probable cyp142, cytochrome P450 monoxygenase (EC
                     1.14.-.-), member of Cytochrome P450 family and similar to
                     many e.g. Q9L465|CYP162A1|NIKQ from Streptomyces tendae
                     (396 aa) FASTA scores: opt: 798, E(): 2e-43, (36.7%
                     identity in 403 aa overlap); P33271|CPXK_SACER|CYP107B1
                     from Saccharopolyspora erythraea (Streptomyces erythraeus)
                     (405 aa), FASTA scores: opt: 725, E(): 9.1e-39, (37.1%
                     identity in 407 aa overlap); Q9X8Q3|CYP107P1|SCH10.14c
                     from Streptomyces coelicolor (411 aa), FASTA scores: opt:
                     691, E(): 1.3e-36, (37.2% identity in 317 aa overlap);
                     etc. Also similar to
                     Q50696|C124_MYCTU|CYP124|Rv2266|MT2328|MTCY339.44c from
                     Mycobacterium tuberculosis strain H37Rv (428 aa) FASTA
                     scores: opt: 692, E(): 1.2e-36, (36.8% identity in 402 aa
                     overlap). Equivalent to AAK47979 from Mycobacterium
                     tuberculosis strain CDC1551 (372 aa) but longer 26 aa.
                     Contains PS00086 Cytochrome P450 cysteine heme-iron ligand
                     signature. BELONGS TO THE CYTOCHROME P450 FAMILY.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, cyp142 exists as a single gene.
                     In Mycobacterium bovis, a frameshift due to a single base
                     deletion (c-*) splits cyp142 into 2 parts, cyp142a and
                     cyp142b. Protein product from Mb3547c detected using SWATH
                     mass spectrometry. Mb3547c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y4F0"
                     /db_xref="InterPro:IPR001128"
                     /db_xref="InterPro:IPR002397"
                     /db_xref="InterPro:IPR017972"
                     /db_xref="InterPro:IPR036396"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y4F0"
BEGIN
        1 MSSEVDGERL SDDELVMETL LILIGGDETT RHTLSGGTEQ LLRNRDQWDL LQRDPSLLPG
       61 AIEEMLRWTA PVKNMCRVLT ADTEFHGTAL CAGEKMMLLF ESANFDEAVF CEPEKFDVQR
      121 NPNSHLAFGF GTHFCLGNQL ARLELSLMTE RVLRRLPDLR LVADDSVLPL RPANFVSGLE
      181 SMPVVFTPSP PLG
//