LOCUS SIU02174.1 193 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CYTOCHROME P450 MONOOXYGENASE 142 CYP142B [SECOND PART] protein. ACCESSION LT708304-3588 PROTEIN_ID SIU02174.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cyp142b" /locus_tag="BQ2027_MB3547C" /note="Mb3547c, cyp142b, len: 193 aa. Equivalent to 3' end of Rv3518c, len: 398 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 193 aa overlap). Probable cyp142, cytochrome P450 monoxygenase (EC 1.14.-.-), member of Cytochrome P450 family and similar to many e.g. Q9L465|CYP162A1|NIKQ from Streptomyces tendae (396 aa) FASTA scores: opt: 798, E(): 2e-43, (36.7% identity in 403 aa overlap); P33271|CPXK_SACER|CYP107B1 from Saccharopolyspora erythraea (Streptomyces erythraeus) (405 aa), FASTA scores: opt: 725, E(): 9.1e-39, (37.1% identity in 407 aa overlap); Q9X8Q3|CYP107P1|SCH10.14c from Streptomyces coelicolor (411 aa), FASTA scores: opt: 691, E(): 1.3e-36, (37.2% identity in 317 aa overlap); etc. Also similar to Q50696|C124_MYCTU|CYP124|Rv2266|MT2328|MTCY339.44c from Mycobacterium tuberculosis strain H37Rv (428 aa) FASTA scores: opt: 692, E(): 1.2e-36, (36.8% identity in 402 aa overlap). Equivalent to AAK47979 from Mycobacterium tuberculosis strain CDC1551 (372 aa) but longer 26 aa. Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. BELONGS TO THE CYTOCHROME P450 FAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, cyp142 exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base deletion (c-*) splits cyp142 into 2 parts, cyp142a and cyp142b. Protein product from Mb3547c detected using SWATH mass spectrometry. Mb3547c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y4F0" /db_xref="InterPro:IPR001128" /db_xref="InterPro:IPR002397" /db_xref="InterPro:IPR017972" /db_xref="InterPro:IPR036396" /db_xref="UniProtKB/TrEMBL:A0A1R3Y4F0" BEGIN 1 MSSEVDGERL SDDELVMETL LILIGGDETT RHTLSGGTEQ LLRNRDQWDL LQRDPSLLPG 61 AIEEMLRWTA PVKNMCRVLT ADTEFHGTAL CAGEKMMLLF ESANFDEAVF CEPEKFDVQR 121 NPNSHLAFGF GTHFCLGNQL ARLELSLMTE RVLRRLPDLR LVADDSVLPL RPANFVSGLE 181 SMPVVFTPSP PLG //