LOCUS SIU02167.1 278 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Amidohydrolase family protein protein. ACCESSION LT708304-3581 PROTEIN_ID SIU02167.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3540C" /note="Mb3540c, -, len: 278 aa. Equivalent to Rv3510c, len: 278 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 278 aa overlap). Conserved hypothetical protein, similar to Q50662|Rv2303c|MTCY339.06 HYPOTHETICAL 34.6 KDA PROTEIN from Mycobacterium tuberculosis (307 aa), FASTA scores: opt: 416, E(): 1.2e-19, (35.7% identity in 255 aa overlap). Middle of the putative protein highly similar to N-terminal end of Q49860|B229_C2_182 HYPOTHETICAL 11.0 KDA PROTEIN from Mycobacterium leprae (95 aa), FASTA scores: opt: 304, E(): 7.9e-13, (83.65% identity in 55 aa overlap). Also some similarity with other bacterial proteins e.g. P95886 ORF C02006 from Sulfolobus solfataricus (269 aa), FASTA scores: opt: 293, E(): 9.6e-12, (31.3% identity in 198 aa overlap); Q9XDF3|NONC NONC PROTEIN from Streptomyces griseus subsp. griseus (317 aa), FASTA scores: opt: 270, E(): 3.4e-10, (29.95% identity in 227 aa overlap); Q54229|NONR MACROTETROLIDE ANTIBIOTIC-RESISTANCE PROTEIN from Streptomyces griseus (347 aa), FASTA scores: opt: 270, E(): 3.6e-10, (29.95% identity in 227 aa overlap); etc. Protein product from Mb3540c detected using SWATH mass spectrometry. Mb3540c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y4C3" /db_xref="InterPro:IPR006680" /db_xref="InterPro:IPR032465" /db_xref="InterPro:IPR032466" /db_xref="UniProtKB/TrEMBL:A0A1R3Y4C3" BEGIN 1 MTIDVWMQHP TQRFLHGDMF ASLRRWTGGS IPETDIPIEA TVSSMDAGGV TLGLLSAWRG 61 PNGQDLISND AVAEWVRLYP NRFAGLAAVD LDRPMAAVRE LRRRVGEGFV GLRVVPWLWG 121 APPTDRRYYP LFAECVQSAV PFCTQVGHTG PLRPSETGRP IPYIDQVALD FPELVIVCGH 181 VGYPWTEEMV AVARKHENVY IDTSAYTIKR LPGKLVRFMK TDTGQRKVLF GTNYPMIAHT 241 HALTGLDELG LSDEARRDFL HGNAVRVFKL DPRGKVQT //