LOCUS       SIU02147.1               500 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 alpha,
            alpha-trehalose-phosphate synthase [udp-forming] otsa
            (trehalose-6-phosphate synthase) (udp-glucose-glucosephosphate
            glucosyltransferase) (trehalosephosphate-udp glucosyltransferase)
            (trehalose-6-phosphate synthetase) (trehalose-phosphate
            synthase) (trehalose-phosphate synthetase) (transglucosylase)
            (trehalosephosphate-udp glucosyl transferase) protein.
ACCESSION   LT708304-3561
PROTEIN_ID  SIU02147.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="otsA"
                     /locus_tag="BQ2027_MB3520"
                     /note="Mb3520, otsA, len: 500 aa. Equivalent to Rv3490,
                     len: 500 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 500 aa overlap). Probable otsA, alpha,
                     alpha-trehalose-phosphate synthase (EC 2.4.1.15) (see
                     citations below), equivalent to Q50167|OTSA|ML2254
                     PROBABLE TREHALOSE-PHOSPHATE SYNTHASE from Mycobacterium
                     leprae (498 aa), FASTA scores: opt: 2706, E(): 1.6e-166,
                     (80.3% identity in 497 aa overlap). Also similar to others
                     e.g. Q92410|TPS1_CANAL from Candida albicans (Yeast) (478
                     aa), FASTA scores: opt: 895, E(): 4.9e-50, (37.15%
                     identity in 479 aa overlap);
                     Q00764|TPS1_YEASTTPS1|CIF1|BYP1|FDP1|GGS1|GLC6|YBR126c|YBR
                     0922 from Saccharomyces cerevisiae (Baker's yeast) (495
                     aa), FASTA scores: opt: 847, E(): 6.2e-47, (36.1% identity
                     in 490 aa overlap); BAB48232|MLL0691 from Rhizobium loti
                     (Mesorhizobium loti) (520 aa), FASTA scores: opt: 884,
                     E(): 2.7e-49, (36.2% identity in 478 aa overlap); etc.
                     Equivalent to AAK47953 from Mycobacterium tuberculosis
                     strain CDC1551 (478 aa) but longer 22 aa. Protein product
                     from Mb3520 detected using shotgun mass spectrometry and
                     SWATH mass spectrometry. Mb3520 found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:Q7TWE1"
                     /db_xref="InterPro:IPR001830"
                     /db_xref="UniProtKB/Swiss-Prot:Q7TWE1"
BEGIN
        1 MAPSGGQEAQ ICDSETFGDS DFVVVANRLP VDLERLPDGS TTWKRSPGGL VTALEPVLRR
       61 RRGAWVGWPG VNDDGAEPDL HVLDGPIIQD ELELHPVRLS TTDIAQYYEG FSNATLWPLY
      121 HDVIVKPLYH REWWDRYVDV NQRFAEAASR AAAHGATVWV QDYQLQLVPK MLRMLRPDLT
      181 IGFFLHIPFP PVELFMQMPW RTEIIQGLLG ADLVGFHLPG GAQNFLILSR RLVGTDTSRG
      241 TVGVRSRFGA AVLGSRTIRV GAFPISVDSG ALDHAARDRN IRRRAREIRT ELGNPRKILL
      301 GVDRLDYTKG IDVRLKAFSE LLAEGRVKRD DTVLVQLATP SRERVESYQT LRNDIERQVG
      361 HINGEYGEVG HPVVHYLHRP APRDELIAFF VASDVMLVTP LRDGMNLVAK EYVACRSDLG
      421 GALVLSEFTG AAAELRHAYL VNPHDLEGVK DGIEEALNQT EEAGRRRMRS LRRQVLAHDV
      481 DRWAQSFLDA LAGAHPRGQG
//