LOCUS SIU02147.1 500 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 alpha, alpha-trehalose-phosphate synthase [udp-forming] otsa (trehalose-6-phosphate synthase) (udp-glucose-glucosephosphate glucosyltransferase) (trehalosephosphate-udp glucosyltransferase) (trehalose-6-phosphate synthetase) (trehalose-phosphate synthase) (trehalose-phosphate synthetase) (transglucosylase) (trehalosephosphate-udp glucosyl transferase) protein. ACCESSION LT708304-3561 PROTEIN_ID SIU02147.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="otsA" /locus_tag="BQ2027_MB3520" /note="Mb3520, otsA, len: 500 aa. Equivalent to Rv3490, len: 500 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 500 aa overlap). Probable otsA, alpha, alpha-trehalose-phosphate synthase (EC 2.4.1.15) (see citations below), equivalent to Q50167|OTSA|ML2254 PROBABLE TREHALOSE-PHOSPHATE SYNTHASE from Mycobacterium leprae (498 aa), FASTA scores: opt: 2706, E(): 1.6e-166, (80.3% identity in 497 aa overlap). Also similar to others e.g. Q92410|TPS1_CANAL from Candida albicans (Yeast) (478 aa), FASTA scores: opt: 895, E(): 4.9e-50, (37.15% identity in 479 aa overlap); Q00764|TPS1_YEASTTPS1|CIF1|BYP1|FDP1|GGS1|GLC6|YBR126c|YBR 0922 from Saccharomyces cerevisiae (Baker's yeast) (495 aa), FASTA scores: opt: 847, E(): 6.2e-47, (36.1% identity in 490 aa overlap); BAB48232|MLL0691 from Rhizobium loti (Mesorhizobium loti) (520 aa), FASTA scores: opt: 884, E(): 2.7e-49, (36.2% identity in 478 aa overlap); etc. Equivalent to AAK47953 from Mycobacterium tuberculosis strain CDC1551 (478 aa) but longer 22 aa. Protein product from Mb3520 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3520 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TWE1" /db_xref="InterPro:IPR001830" /db_xref="UniProtKB/Swiss-Prot:Q7TWE1" BEGIN 1 MAPSGGQEAQ ICDSETFGDS DFVVVANRLP VDLERLPDGS TTWKRSPGGL VTALEPVLRR 61 RRGAWVGWPG VNDDGAEPDL HVLDGPIIQD ELELHPVRLS TTDIAQYYEG FSNATLWPLY 121 HDVIVKPLYH REWWDRYVDV NQRFAEAASR AAAHGATVWV QDYQLQLVPK MLRMLRPDLT 181 IGFFLHIPFP PVELFMQMPW RTEIIQGLLG ADLVGFHLPG GAQNFLILSR RLVGTDTSRG 241 TVGVRSRFGA AVLGSRTIRV GAFPISVDSG ALDHAARDRN IRRRAREIRT ELGNPRKILL 301 GVDRLDYTKG IDVRLKAFSE LLAEGRVKRD DTVLVQLATP SRERVESYQT LRNDIERQVG 361 HINGEYGEVG HPVVHYLHRP APRDELIAFF VASDVMLVTP LRDGMNLVAK EYVACRSDLG 421 GALVLSEFTG AAAELRHAYL VNPHDLEGVK DGIEEALNQT EEAGRRRMRS LRRQVLAHDV 481 DRWAQSFLDA LAGAHPRGQG //