LOCUS SIU02142.1 314 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE protein. ACCESSION LT708304-3556 PROTEIN_ID SIU02142.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3515C" /note="Mb3515c, -, len: 314 aa. Equivalent to Rv3485c, len: 314 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 314 aa overlap). Probable short-chain dehydrogenase/reductase (EC 1.-.-.-), similar, but longer 41 aa, to P71824|Rv0769|MTCY369.14 PUTATIVE SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE CY369.14 from Mycobacterium tuberculosis (248 aa), FASTA scores: opt: 462, E(): 1.8e-19, (34.0% identity in 253 aa overlap). Also similar to various dehydrogenases e.g. P25529|HDHA_ECOLI|HSDH|B1619 NAD-DEPENDENT 7 ALPHA-HYDROXYSTEROID DEHYDROGENASE (SDR FAMILY) (EC 1.1.1.159) from Escherichia coli strain K12 (alias BAB35750|ECS2327 or AAG56608|HDHA for strain O157:H7) (255 aa), FASTA scores: opt: 462, E(): 1.8e-19, (34.7% identity in 248 aa overlap); Q9FD15|RUBG PUTATIVE REDUCTASE (SDR FAMILY) from Streptomyces collinus (249 aa), FASTA scores: opt: 446, E(): 1.5e-18, (36.1% identity in 255 aa overlap); BAB51974|MLL5540 PUTATIVE DEHYDROGENASE from Rhizobium loti (Mesorhizobium loti) (253 aa), FASTA scores: opt: 442, E(): 2.5e-18, (36.25% identity in 251 aa overlap); Q08632|SDR1_PICAB SHORT-CHAIN TYPE DEHYDROGENASE/REDUCTASE (SDR FAMILY) from Picea abies (Norway spruce) (Picea excelsa) (271 aa), FASTA scores: opt: 441, E(): 3.1e-18, (32.3% identity in 260 aa overlap); Q9A326|CC3380 2-DEOXY-D-GLUCONATE 3-DEHYDROGENASE from Caulobacter crescentus (260 aa), FASTA scores: opt: 436, E(): 5.7e-18, (32.8% identity in 253 aa overlap); Q16698|DECR_HUMAN 2,4-DIENOYL-COA REDUCTASE, MITOCHONDRIAL PRECURSOR (EC 1.3.1.34) from Homo sapiens (Human) (335 aa), FASTA scores: opt: 430, E(): 1.5e-17, (30.4% identity in 306 aa overlap); etc. Contains short-chain alcohol dehydrogenase family signature (PS00061). BELONGS TO THE SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY (SDR). Protein product from Mb3515c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3515c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y4D9" /db_xref="InterPro:IPR002347" /db_xref="InterPro:IPR020904" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3Y4D9" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNSRAPRNLA VSSPSAQVTG RMVQNGENLF QFRREGPQVQ LSFQDRTYLV TGGGSGIGKG 61 VAAGLVAAGA AVMIVGRNPD KLAAAVKDIE ALKTGAIGYE PADITDEEQT LRVVDAATAW 121 HGRLHGVVHC AGGSQTIGPI TQIDSQAWRR TVDLNVNGTM YVLKHAAREL VRGGGGSFVG 181 ISSIAASNTH RWFGAYGVTK SAVDHMMKLA ADELGPSWVR VNSIRPGLIR TDLVVPVTES 241 PELSADYRVC TPLPRVGEVE DVANLAMFLL SDAASWITGQ VINVDGGHML RRGPDFSPML 301 EPVFGADGLR GVVG //