LOCUS SIU02126.1 336 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE 4-HYDROXY-2-OXOVALERATE ALDOLASE MHPE (HOA) protein. ACCESSION LT708304-3540 PROTEIN_ID SIU02126.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mhpE" /locus_tag="BQ2027_MB3498C" /note="Mb3498c, mhpE, len: 336 aa. Equivalent to Rv3469c, len: 336 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 336 aa overlap). Probable mhpE, 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.-), similar to others (principally from Pseudomonas species) e.g. Q99PZ1|SCP1.301|SCP1.53c from Streptomyces coelicolor (338 aa), FASTA scores: opt: 615, E(): 7.9e-31, (37.65% identity in 332 aa overlap); Q9X9Q0|NIKB NIKB PROTEIN (see first citation below) from Streptomyces tendae (357 aa), FASTA scores: opt: 571, E(): 4.4e-28, (34.5% identity in 339 aa overlap); P51014|BPHF_PSES1 from Pseudomonas sp. strain KKS102 (352 aa), FASTA scores: opt: 549, E(): 9.9e-27, (31.2% identity in 314 aa overlap); Q51983|CMTG_PSEPU from Pseudomonas putida (350 aa), FASTA scores: opt: 543, E(): 2.3e-26, (30.7% identity in 319 aa overlap); P51020|MHPE_ECOLI|MHPF|B0352 from Escherichia coli strain K12 (337 aa), FASTA scores: opt: 517, E(): 9.1e-25, (31.75% identity in 312 aa overlap); etc. Also similar to P71867|MTCY03C7.22|Rv3534c HYPOTHETICAL 36.4 KDA PROTEIN from Mycobacterium tuberculosis (346 aa), FASTA scores: E(): 7.5e-24, (31.9% identity in 310 aa overlap). Mb3498c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y4Y7" /db_xref="InterPro:IPR000891" /db_xref="InterPro:IPR013785" /db_xref="UniProtKB/TrEMBL:A0A1R3Y4Y7" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MLMTATHREP IVLDTTVRDG SYAVNFQYTD DDVRRIVGDL DAAGIPYIEI GHGVTIGAAA 61 AQGPAAHTDE EYFRAARSVV RNARLGAVIV PALARIETVD LAGDYLDFLR ICVIATEFEL 121 VMPFVERAQS KGLEVSIQLV KSHLFEPDVL AAAGKRARDV GVRIVYVVDT TGTFLPEDAR 181 RYVEALRGAS DVSVGFHGHN NLAMAVANTL EAFDAGADFL DGTLMGFGRG AGNCQIECLV 241 AALQRRGHLA AVDLDRIFDA ARSDMLGRSP QSYGIDPWEI SFGFHGLDSL QVEHLRAAAQ 301 QAGLSVSHVI RQTAKSHAGQ WLSPQDIDRV VVGMRA //