LOCUS       SIU02114.1               347 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            DNA-DIRECTED RNA POLYMERASE (ALPHA CHAIN) RPOA (TRANSCRIPTASE
            ALPHA CHAIN) (RNA POLYMERASE ALPHA SUBUNIT) (DNA-DIRECTED
            RNA NUCLEOTIDYLTRANSFERASE) protein.
ACCESSION   LT708304-3528
PROTEIN_ID  SIU02114.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="rpoA"
                     /locus_tag="BQ2027_MB3486C"
                     /note="Mb3486c, rpoA, len: 347 aa. Equivalent to Rv3457c,
                     len: 347 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 347 aa overlap). Probable rpoA, alpha
                     chain of RNA polymerase (EC 2.7.7.6), equivalent to
                     Q9X798|RPOA_MYCLE|ML1957|MLCB1222.27c DNA-DIRECTED RNA
                     POLYMERASE ALPHA from Mycobacterium leprae (347 aa), FASTA
                     scores: opt: 2139, E(): 1.3e-123, (95.65% identity in 347
                     aa overlap). Also highly similar to others e.g.
                     P72404|RPOA_STRCO|C6G4.07 from Streptomyces coelicolor
                     (340 aa), FASTA scores: opt: 1672, E(): 4.7e-95, (75.55%
                     identity in 348 aa overlap); Q9X4V6|RPOA_STRGT from
                     Streptomyces granaticolor (340 aa), FASTA scores: opt:
                     1671, E(): 5.4e-95, (75.55% identity in 348 aa overlap);
                     P20429|RPOA_BACSU from Bacillus subtilis (314 aa), FASTA
                     scores: opt: 939, E(): 3e-50, (48.9% identity in 311 aa
                     overlap); etc. Contains (PS00017) ATP/GTP-binding site
                     motif A (P-loop). BELONGS TO THE RNA POLYMERASE ALPHA
                     CHAIN FAMILY. Protein product from Mb3486c detected using
                     shotgun mass spectrometry and SWATH mass spectrometry.
                     Mb3486c found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing."
                     /db_xref="GOA:P66702"
                     /db_xref="InterPro:IPR011260"
                     /db_xref="InterPro:IPR011262"
                     /db_xref="InterPro:IPR011263"
                     /db_xref="InterPro:IPR011773"
                     /db_xref="InterPro:IPR036603"
                     /db_xref="InterPro:IPR036643"
                     /db_xref="UniProtKB/Swiss-Prot:P66702"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MLISQRPTLS EDVLTDNRSQ FVIEPLEPGF GYTLGNSLRR TLLSSIPGAA VTSIRIDGVL
       61 HEFTTVPGVK EDVTEIILNL KSLVVSSEED EPVTMYLRKQ GPGEVTAGDI VPPAGVTVHN
      121 PGMHIATLND KGKLEVELVV ERGRGYVPAV QNRASGAEIG RIPVDSIYSP VLKVTYKVDA
      181 TRVEQRTDFD KLILDVETKN SISPRDALAS AGKTLVELFG LARELNVEAE GIEIGPSPAE
      241 ADHIASFALP IDDLDLTVRS YNCLKREGVH TVGELVARTE SDLLDIRNFG QKSIDEVKIK
      301 LHQLGLSLKD SPPSFDPSEV AGYDVATGTW STEGAYDEQD YAETEQL
//