LOCUS SIU02114.1 347 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE DNA-DIRECTED RNA POLYMERASE (ALPHA CHAIN) RPOA (TRANSCRIPTASE ALPHA CHAIN) (RNA POLYMERASE ALPHA SUBUNIT) (DNA-DIRECTED RNA NUCLEOTIDYLTRANSFERASE) protein. ACCESSION LT708304-3528 PROTEIN_ID SIU02114.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="rpoA" /locus_tag="BQ2027_MB3486C" /note="Mb3486c, rpoA, len: 347 aa. Equivalent to Rv3457c, len: 347 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 347 aa overlap). Probable rpoA, alpha chain of RNA polymerase (EC 2.7.7.6), equivalent to Q9X798|RPOA_MYCLE|ML1957|MLCB1222.27c DNA-DIRECTED RNA POLYMERASE ALPHA from Mycobacterium leprae (347 aa), FASTA scores: opt: 2139, E(): 1.3e-123, (95.65% identity in 347 aa overlap). Also highly similar to others e.g. P72404|RPOA_STRCO|C6G4.07 from Streptomyces coelicolor (340 aa), FASTA scores: opt: 1672, E(): 4.7e-95, (75.55% identity in 348 aa overlap); Q9X4V6|RPOA_STRGT from Streptomyces granaticolor (340 aa), FASTA scores: opt: 1671, E(): 5.4e-95, (75.55% identity in 348 aa overlap); P20429|RPOA_BACSU from Bacillus subtilis (314 aa), FASTA scores: opt: 939, E(): 3e-50, (48.9% identity in 311 aa overlap); etc. Contains (PS00017) ATP/GTP-binding site motif A (P-loop). BELONGS TO THE RNA POLYMERASE ALPHA CHAIN FAMILY. Protein product from Mb3486c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3486c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P66702" /db_xref="InterPro:IPR011260" /db_xref="InterPro:IPR011262" /db_xref="InterPro:IPR011263" /db_xref="InterPro:IPR011773" /db_xref="InterPro:IPR036603" /db_xref="InterPro:IPR036643" /db_xref="UniProtKB/Swiss-Prot:P66702" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MLISQRPTLS EDVLTDNRSQ FVIEPLEPGF GYTLGNSLRR TLLSSIPGAA VTSIRIDGVL 61 HEFTTVPGVK EDVTEIILNL KSLVVSSEED EPVTMYLRKQ GPGEVTAGDI VPPAGVTVHN 121 PGMHIATLND KGKLEVELVV ERGRGYVPAV QNRASGAEIG RIPVDSIYSP VLKVTYKVDA 181 TRVEQRTDFD KLILDVETKN SISPRDALAS AGKTLVELFG LARELNVEAE GIEIGPSPAE 241 ADHIASFALP IDDLDLTVRS YNCLKREGVH TVGELVARTE SDLLDIRNFG QKSIDEVKIK 301 LHQLGLSLKD SPPSFDPSEV AGYDVATGTW STEGAYDEQD YAETEQL //