LOCUS       SIU02112.1               256 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            TRNA PSEUDOURIDINE SYNTHASE A TRUA (PSEUDOURIDYLATE SYNTHASE
            I) (PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE) protein.
ACCESSION   LT708304-3526
PROTEIN_ID  SIU02112.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="truA"
                     /locus_tag="BQ2027_MB3484C"
                     /note="Mb3484c, truA, len: 256 aa. Equivalent to Rv3455c,
                     len: 256 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 256 aa overlap). Probable truA,
                     pseudouridine synthase A (EC 4.2.1.70), equivalent to
                     Q9X796|TRUA_MYCLE|ML1955|MLCB1222.25c TRNA PSEUDOURIDINE
                     SYNTHASE A from Mycobacterium leprae (249 aa), FASTA
                     scores: opt: 1345, E(): 3.2e-80, (77.25% identity in 246
                     aa overlap). Also highly similar to others e.g.
                     O86776|TRUA_STRCO|SC6G4.09 from Streptomyces coelicolor
                     (284 aa), FASTA scores: opt: 595, E(): 1.7e-31, (49.8%
                     identity in 259 aa overlap); Q9RS37|DR2290 from
                     Deinococcus radiodurans (280 aa), FASTA scores: opt: 383,
                     E(): 1e-17, (41.2% identity in 216 aa overlap);
                     Q9PJT0|TRUA_CHLMU|TC0748 from Chlamydia muridarum (267
                     aa), FASTA scores: opt: 334, E(): 1.5e-14, (37.65%
                     identity in 231 aa overlap);
                     P07649|TRUA_ECOLI|HIST|ASUC|LEUK|B2318 from Escherichia
                     coli strain K12 (270 aa), FASTA scores: opt: 315, E():
                     2.5e-13, (33.35% identity in 240 aa overlap); etc. BELONGS
                     TO THE TRUA FAMILY OF PSEUDOURIDINE SYNTHASES. Protein
                     product from Mb3484c detected using SWATH mass
                     spectrometry. Mb3484c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P65847"
                     /db_xref="InterPro:IPR001406"
                     /db_xref="InterPro:IPR020095"
                     /db_xref="InterPro:IPR020097"
                     /db_xref="InterPro:IPR020103"
                     /db_xref="UniProtKB/Swiss-Prot:P65847"
BEGIN
        1 MGQRTVAGDL DAALTTIFRT PVRLRAAGRT DAGVHASGQV AHVDVPADAL PNAYPRAGHV
       61 GDPEFLPLLR RLGRFLPADV RILDITRAPA GFDARFSALR RHYVYRLSTA PYGVEPQQAR
      121 YITAWPRELD LDAMTAASRD LMGLHDFAAF CRHREGATTI RDLQRLDWSR AGTLVTAHVT
      181 ADAFCWSMVR SLVGALLAVG EHRRATTWCR ELLTATGRSS DFAVAPAHGL TLIQVDYPPD
      241 DQLASRNLVT RDVRSG
//