LOCUS SIU02112.1 256 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE TRNA PSEUDOURIDINE SYNTHASE A TRUA (PSEUDOURIDYLATE SYNTHASE I) (PSEUDOURIDINE SYNTHASE I) (URACIL HYDROLYASE) protein. ACCESSION LT708304-3526 PROTEIN_ID SIU02112.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="truA" /locus_tag="BQ2027_MB3484C" /note="Mb3484c, truA, len: 256 aa. Equivalent to Rv3455c, len: 256 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 256 aa overlap). Probable truA, pseudouridine synthase A (EC 4.2.1.70), equivalent to Q9X796|TRUA_MYCLE|ML1955|MLCB1222.25c TRNA PSEUDOURIDINE SYNTHASE A from Mycobacterium leprae (249 aa), FASTA scores: opt: 1345, E(): 3.2e-80, (77.25% identity in 246 aa overlap). Also highly similar to others e.g. O86776|TRUA_STRCO|SC6G4.09 from Streptomyces coelicolor (284 aa), FASTA scores: opt: 595, E(): 1.7e-31, (49.8% identity in 259 aa overlap); Q9RS37|DR2290 from Deinococcus radiodurans (280 aa), FASTA scores: opt: 383, E(): 1e-17, (41.2% identity in 216 aa overlap); Q9PJT0|TRUA_CHLMU|TC0748 from Chlamydia muridarum (267 aa), FASTA scores: opt: 334, E(): 1.5e-14, (37.65% identity in 231 aa overlap); P07649|TRUA_ECOLI|HIST|ASUC|LEUK|B2318 from Escherichia coli strain K12 (270 aa), FASTA scores: opt: 315, E(): 2.5e-13, (33.35% identity in 240 aa overlap); etc. BELONGS TO THE TRUA FAMILY OF PSEUDOURIDINE SYNTHASES. Protein product from Mb3484c detected using SWATH mass spectrometry. Mb3484c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65847" /db_xref="InterPro:IPR001406" /db_xref="InterPro:IPR020095" /db_xref="InterPro:IPR020097" /db_xref="InterPro:IPR020103" /db_xref="UniProtKB/Swiss-Prot:P65847" BEGIN 1 MGQRTVAGDL DAALTTIFRT PVRLRAAGRT DAGVHASGQV AHVDVPADAL PNAYPRAGHV 61 GDPEFLPLLR RLGRFLPADV RILDITRAPA GFDARFSALR RHYVYRLSTA PYGVEPQQAR 121 YITAWPRELD LDAMTAASRD LMGLHDFAAF CRHREGATTI RDLQRLDWSR AGTLVTAHVT 181 ADAFCWSMVR SLVGALLAVG EHRRATTWCR ELLTATGRSS DFAVAPAHGL TLIQVDYPPD 241 DQLASRNLVT RDVRSG //