LOCUS SIU02107.1 455 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable
membrane-anchored mycosin mycp4 (serine protease) (subtilisin-
like protease) (subtilase-like) (mycosin-4) protein.
ACCESSION LT708304-3521
PROTEIN_ID SIU02107.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="mycp4"
/locus_tag="BQ2027_MB3479"
/note="Mb3479, -, len: 455 aa. Equivalent to Rv3449, len:
455 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 455 aa overlap). Probable secreted
serine protease (EC 3.4.21.-). Similar to hypothetical
unknowns or proteases from Mycobacterium tuberculosis
strains H37Rv and CDC1551 e.g. AAK48366|MT3998 SUBTILASE
FAMILY PROTEIN from Mycobacterium tuberculosis strain
CDC1551 (411 aa), FASTA scores: opt: 747, E(): 3.5e-33,
(45.65% identity in 416 aa overlap);
O05461|Rv3883c|MTCY15F10.29 HYPOTHETICAL PROTEIN (446 aa),
FASTA scores: opt: 747, E(): 3.8e-33, (45.45% identity in
451 aa overlap); O53695|Rv0291|MTV035.19 HYPOTHETICAL
PROTEIN (461 aa), FASTA scores: opt: 660, E(): 1.9e-28,
(44.0% identity in 457 aa overlap); etc. And similar to
hypothetical proteases from Mycobacterium leprae e.g.
O33076|MLCB628.04|ML0041 HYPOTHETICAL 45.7 KDA PROTEIN
(PROBABLE SECRETED PROTEASE) (446 aa), FASTA scores: opt:
683, E(): 1.1e-29, (43.8% identity in 450 aa overlap);
Q9CD36|ML2528 PUTATIVE PROTEASE (475 aa), FASTA scores:
opt: 608, E(): 1.3e-25, (43.0% identity in 451 aa
overlap); Q9CBV3|ML1538 POSSIBLE PROTEASE (567 aa), FASTA
scores: opt: 389, E(): 9.7e-14, (33.8% identity in 562 aa
overlap); etc. Also some similarity to other proteases
from several organisms e.g. O31788|APRX ALKALINE SERINE
PROTEASE from Bacillus subtilis (442 aa), FASTA scores:
opt: 296, E(): 8.3e-09, (29.4% identity in 313 aa
overlap); O86650|SC3C3.17c PUTATIVE SECRETED SERINE
PROTEASE from Streptomyces coelicolor (450 aa), FASTA
scores: opt: 279, E(): 7e-08, (33.55% identity in 343 aa
overlap); Q9KBJ7|APRX|BH193 INTRACELLULAR ALKALINE SERINE
PROTEASE from Bacillus halodurans (444 aa), FASTA scores:
opt: 257, E(): 1.1e-06, (28.65% identity in 335 aa
overlap); O86642|SC3C3.08 SERINE PROTEASE from
Streptomyces coelicolor (413 aa), FASTA scores: opt: 243,
E(): 5.7e-06, (38.25% identity in 387 aa overlap); etc.
Has putative signal peptide at N-terminus and hydrophobic
stretch at C-terminus. Contains three signatures typical
of subtilase family: aspartic acid active site (PS00136),
histidine active site (PS00137), serine active site
(PS00138)."
/db_xref="GOA:A0A1R3Y468"
/db_xref="InterPro:IPR000209"
/db_xref="InterPro:IPR015500"
/db_xref="InterPro:IPR022398"
/db_xref="InterPro:IPR023827"
/db_xref="InterPro:IPR023828"
/db_xref="InterPro:IPR023834"
/db_xref="InterPro:IPR036852"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y468"
BEGIN
1 MTTSRTLRLL VVSALATLSG LGTPVAHAVS PPPIDERWLP ESALPAPPRP TVQREVCTEV
61 TAESGRAFGR AERSAQLADL DQVWRLTRGA GQRVAVIDTG VARHRRLPKV VAGGDYVFTG
121 DGTADCDAHG TLVAGIIAAA PDAQSDNFSG VAPDVTLISI RQSSSKFAPV GDPSSTGVGD
181 VDTMAKAVRT AADLGASVIN ISSIACVPAA AAPDDRALGA ALAYAVDVKN AVIVAAAGNT
241 GGAAQCPPQA PGVTRDSVTV AVSPAWYDDY VLTVGSVNAQ GEPSAFTLAG PWVDVAATGE
301 AVTSLSPFGD GTVNRLGGQH GSIPISGTSY AAPVVSGLAA LIRARFPTLT ARQVMQRIES
361 TAHHPPAGWD PLVGNGTVDA LAAVSSDSIP QAGTATSDPA PVAVPVPRRS TPGPSDRRAL
421 HTAFAGAAIC LLALMATLAT ASRRLRPGRN GIAGD
//