LOCUS SIU02077.1 275 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CONSERVED HYPOTHETICAL PROTEIN protein. ACCESSION LT708304-3491 PROTEIN_ID SIU02077.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3449C" /note="Mb3449c, -, len: 275 aa. Equivalent to Rv3415c, len: 275 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 275 aa overlap). Conserved hypothetical protein, equivalent to Q9CCV3|ML0383 HYPOTHETICAL PROTEIN from Mycobacterium leprae (281 aa), FASTA scores: opt: 1278, E(): 4.2e-71, (73.5% identity in 279 aa overlap); and Q49858|B229_C1_175 HYPOTHETICAL 27.4 KDA PROTEIN from Mycobacterium leprae (264 aa), FASTA scores: opt: 1186, E(): 1.7e-65, (74.05% identity in 258 aa overlap). And C-terminus highly similar to N-terminal part of Q49726|B1620_C3_232 HYPOTHETICAL 12.9 KDA PROTEIN from Mycobacterium leprae (122 aa), FASTA scores: opt: 580, E(): 1.1e-28, (74.6% identity in 126 aa overlap). Also some similarity with P71677|RIBD_MYCTU|RIBG|Rv1409|MT1453|MTCY21B4.26 RIBOFLAVIN BIOSYNTHESIS PROTEIN R (339 aa), FASTA scores: opt: 143, E(): 0.13, (28.25% identity in 184 aa overlap). Protein product from Mb3449c detected using SWATH mass spectrometry. Mb3449c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR011990" /db_xref="UniProtKB/TrEMBL:A0A1R3Y441" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNETPHAPVV EQVLVAAAFG NQPGSWPLPT AITPHHLWLR AVAAGGQGRY AHAYGDLSVL 61 RRLVPAGPLA SLAHSTQGSL LRQLGWHTLA RGWDGRALAL AGADREAGAD ALIGLAADAL 121 GVGRFAAAGA LLDRADPLVV SPLVADRLAV RRRWVAAELA MATGDGATAV RHAEEAVELT 181 QAMAVASARH RVKSDVVLAA ALCSAGAVAR ARAVGEEALD ATARFGLLPL RWALACLLID 241 IGTVTFSAQQ LRELTKIRNI CAGQVRRAGG CWRTA //