LOCUS SIU02054.1 308 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE NUCLEOSIDE HYDROLASE IUNH (PURINE NUCLEOSIDASE) protein. ACCESSION LT708304-3468 PROTEIN_ID SIU02054.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="iunH" /locus_tag="BQ2027_MB3425" /note="Mb3425, iunH, len: 308 aa. Equivalent to Rv3393, len: 308 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 308 aa overlap). Probable iunH, nucleoside hydrolase (EC 3.2.2.-), similar to others e.g. Q9RXB2|DR0403 from Deinococcus radiodurans (314 aa), FASTA scores: opt: 497, E(): 6e-24, (34.3% identity in 312 aa overlap); Q27546|IUNH_CRIFA from Crithidia fasciculata (314 aa), FASTA scores: opt: 475, E(): 1.4e-22, (31.45% identity in 318 aa overlap); Q9CK67|IUNH from Pasteurella multocida (310 aa), FASTA scores: opt: 464, E(): 6.9e-22, (30.9% identity in 314 aa overlap); Q9A549|CC2615 from Caulobacter crescentus (323 aa), FASTA scores: opt: 464, E(): 7.2e-22, (37.85% identity in 280 aa overlap); etc. Note that also similar to BAB34113|ECS0690 (alias AAG54985|YBEK) PUTATIVE TRNA SYNTHETASE from Escherichia coli strain O157:H7 (311 aa), FASTA scores: opt: 483, E(): 4.5e-23, (33.0% identity in 315 aa overlap). The active site histidine is conserved. Mb3425 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y430" /db_xref="InterPro:IPR001910" /db_xref="InterPro:IPR023186" /db_xref="InterPro:IPR036452" /db_xref="UniProtKB/TrEMBL:A0A1R3Y430" BEGIN 1 MSVVFADVDT GIDDALAVIY LLASPDADLV GIASTGGNIA VGQVCANNLS LLELCGAADI 61 PVSKGADEPL GGRWPDHPKF HGPKGIGYAE LPASNRRLTD YDATTAWIAA AHSHAGDLIG 121 LVTGPLTNLA LALRAEPALP RLLRRLVIMG GMFDGQPITE WNIRVDPEAA SEVFTAWAGQ 181 RQLPIVCGLD LTRRVAMTPD ILARLASVCG SSPVMRVIED ALRFYFESHE ARGHGYLAYM 241 HDPLAAAVAM DPELLTTRTA TVDVDPTGAT VTDWSGKRNP NARIGMSVDP AVFFDRFVER 301 IGRFARRT //