LOCUS SIU02005.1 1337 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 pe-
pgrs family protein pe_pgrs49 protein.
ACCESSION LT708304-3419
PROTEIN_ID SIU02005.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="PE_PGRS50b"
/locus_tag="BQ2027_MB3376C"
/note="Mb3376c, PE_PGRS50b, len: 1337 aa. Equivalent to
middle part of Rv3345c (PE_PGRS50) and Rv3344c
(PE_PGRS49), len: 1538 aa and 484 aa, from Mycobacterium
tuberculosis strain H37Rv, (81.45% identity in 992 aa
overlap and 100.000% identity in 477 aa overlap). Rv3345c:
Member of the Mycobacterium tuberculosis PE family, PGRS
subfamily of gly-rich proteins. Similar to AAK47791 from
strain CDC1551 but with some big gaps (after residues 501
and 1419; and for AAK47791 after residue 991). Similar to
many from Mycobacterium tuberculosis strains H37Rv and
CDC1551 e.g. O53559|Rv3514|MTV023.21 (1489 aa), FASTA
scores: opt: 4508, E(): 7e-161, (52.1% identity in 1529 aa
overlap); MTV004_1, MTV023_21, MTV023_15, MTCY493_4,
MTV039_16, MTV008_46, MTV023_14, MTV023_19, MTV043_26,
MTCY493_2, MTCY441_4; etc. Rv3344c: Member of the
Mycobacterium tuberculosis PE family, PGRS subfamily of
gly-, ala-rich proteins. Appears to be a gene fragment,
should be in-frame with following ORF, MTV016.45c,
frameshift required around 49595 but could not be found on
checking BAC and cosmid clones. Similar to many from M.
tuberculosis strains H37Rv and CDC1551 e.g.
O53557|Rv3512|MTV023.19 (1079 aa), FASTA scores: opt:
1595, E(): 1.8e-54, (52.0% identity in 544 aa overlap).
REMARK-M.bovis-M.tuberculosis: In Mycobacterium
tuberculosis strain H37Rv, PE_PGRS50 exists as a single
gene. In Mycobacterium bovis, a single base deletion (g-*)
splits PE_PGRS50 into 2 parts, PE_PGRS50a and PE_PGRS50b.
Also in Mycobacterium tuberculosis strain H37Rv, PE_PGRS49
and PE_PGRS50 exist as 2 genes. In Mycobacterium bovis, a
single base deletion (c-*) leads to PE_PGRS49 and
PE_PGRS50b existing as a single product."
/db_xref="UniProtKB/TrEMBL:A0A1R3Y5N3"
BEGIN
1 MTLAVNQGAG GDGGNGGEVG VGGKGGAGGV SANPALNGSA GANGTAPTSG GNGGNGGAGA
61 TPTVAGENGG AGGNGGHGGS VGNGGAGGAG GNGVAGTGLA LNGGNGGIGG NGGSAAGTGG
121 DGGKGGNGGA GANGQDFSAS ANGANGGQGG NGGNGGIGGK GGDAFATFAK AGNGGAGGNG
181 GAAGNGGGGA AGDVTLAINQ GAGGAGGNGG NVGVAGQGGA GGKGAIPAMK GATGADGTAP
241 TSGGDGGNGG NGASPTVAGG NGGDGGKGGS GGNVGNGGNG GAGGNGAAGQ AGTPGPTSGD
301 SGTSGTDGGA GGNGGAGGAG GTLAGHGGNG GKGVNGGQGG IGGAGERGAD GAGPNANGAN
361 GENGGSGGNG GDGGAGGNGG AGGKAQAAGY TDGATGTGGD GGNGGDGGKA GDGGAGANGL
421 NSGAMLPGGG TVGNPGTGGN GGNGGNAGVG GTGGKAGTGS LTGLDGTDGI TPNGGNGGNG
481 GNGGKGGTAG NGSGAAGGNG GNGGSGLNGG DAGNGGNGGG ALNQAGFFGT GGKGGNGGNG
541 GAGMINGGLG GFGGAGGGGA VDVAATTGGA GGNGGAGGFA STGLGGPGGA GGPGGAGDFA
601 SGVGGVGGAG GDGGAGGVGG FGGQGGIGGE GRTGGNGGSG GDGGGGISLG GQGGNGGFGG
661 AGGNGGIGTD AGGAGGAGGA GGNGGSSKST TTGNAGSGGA GGNGGTGLNG AGGAGGAGGN
721 AGVAGVSFGN AVGGDGGNGG NGGHGGDGTT GGAGGKGGNG SSGAASGSGV VNVTAGHGGN
781 GGNGGNSGNS TGVAGLAGGA AGAGGNGGGT SSAAGHGGSG GNGGSGGSGG SGTTGGAGAA
841 GGNGGAGAGG GSLSTGQSGG HGGSGGAGGN GGAGSAGNGG AGGAGGNGGA GGNGGGGDAG
901 NAGSGGNGGK GGDGVGPGST GGAGGKGGAG ANGGSSNGNA RGGNAGNGGH GGAGGSGDTG
961 GAGGAGGQGG FGGTGGSGSG IGGGAGGNGG NGGAGGTGVV LGGKGGDGGN GDHGGPATNP
1021 GSGSRGGAGG SGGNGGAGGN ATGSGGKGGA GGNGGDGSFG ATSGPASIGV TGAPGGNGGK
1081 GGAGGSNPNG SGGDGGKGGN GGAGGNGGSI GANSGIVGGS GGAGGAGGAG GNGSLSSGEG
1141 GKGGDGGHGG DGVGGNSSVT QGGSGGGGGA GGAGGSGFFG GKGGFGGDGG QGGPNGGGTV
1201 GTVAGGGGNG GVGGRGGDGV FAGAGGQGGL GGQGGNGGGS TGGNGGLGGA GGGGGNAPDG
1261 GFGGNGGKGG QGGIGGGTQS ATGLGGDGGD GGDGGNGGNS GAKAGGAGGK GQAGQPNSGT
1321 EPGFGGDGGL GGAGATP
//