LOCUS SIU01998.1 336 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE TRYPTOPHANYL-TRNA SYNTHETASE TRPS (TRYPTOPHAN--TRNA LIGASE) (TRPRS) (TRYPTOPHAN TRANSLASE) protein. ACCESSION LT708304-3412 PROTEIN_ID SIU01998.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="trpS" /locus_tag="BQ2027_MB3369C" /note="Mb3369c, trpS, len: 336 aa. Equivalent to Rv3336c, len: 336 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 336 aa overlap). Probable trpS, tryptophanyl-tRNA synthetase (EC 6.1.1.2), equivalent to Q49901|SYW_MYCLE|TRPS|ML0686|L308_C1_147 TRYPTOPHANYL-TRNA SYNTHETASE from Mycobacterium leprae (343 aa), FASTA scores: opt: 1859, E(): 4.8e-107, (83.75% identity in 339 aa overlap). Also similar to many e.g. Q9KZA7|TRPS2 from Streptomyces coelicolor (339 aa), FASTA scores: opt: 1359, E(): 2.6e-76, (60.3% identity in 335 aa overlap); Q9EYY6|TRPS from Klebsiella aerogenes (334 aa), FASTA scores: opt: 1077, E(): 5.5e-59, (52.15% identity in 328 aa overlap); P00954|SYW_ECOLI|TRPS|B3384 from Escherichia coli strain K12 (334 aa), FASTA scores: opt: 1074, E(): 8.3e-59, (51.85% identity in 328 aa overlap); etc. Contains PS00178 Aminoacyl-transfer RNA synthetases class-I signature. BELONGS TO CLASS-I AMINOACYL-TRNA SYNTHETASE FAMILY. Protein product from Mb3369c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3369c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P67591" /db_xref="InterPro:IPR001412" /db_xref="InterPro:IPR002305" /db_xref="InterPro:IPR002306" /db_xref="InterPro:IPR014729" /db_xref="InterPro:IPR024109" /db_xref="UniProtKB/Swiss-Prot:P67591" BEGIN 1 MSTPTGSRRI FSGVQPTSDS LHLGNALGAV AQWVGLQDDH DAFFCVVDLH AITIPQDPEA 61 LRRRTLITAA QYLALGIDPG RATIFVQSQV PAHTQLAWVL GCFTGFGQAS RMTQFKDKSA 121 RQGSEATTVG LFTYPVLQAA DVLAYDTELV PVGEDQRQHL ELARDVAQRF NSRFPGTLVV 181 PDVLIPKMTA KIYDLQDPTS KMSKSAGTDA GLINLLDDPA LSAKKIRSAV TDSERDIRYD 241 PDVKPGVSNL LNIQSAVTGT DIDVLVDGYA GHGYGDLKKD TAEAVVEFVN PIQARVDELT 301 ADPAELEAVL AAGAQRAHDV ASKTVQRVYD RLGFLL //