LOCUS       SIU01998.1               336 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            TRYPTOPHANYL-TRNA SYNTHETASE TRPS (TRYPTOPHAN--TRNA LIGASE)
            (TRPRS) (TRYPTOPHAN TRANSLASE) protein.
ACCESSION   LT708304-3412
PROTEIN_ID  SIU01998.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="trpS"
                     /locus_tag="BQ2027_MB3369C"
                     /note="Mb3369c, trpS, len: 336 aa. Equivalent to Rv3336c,
                     len: 336 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 336 aa overlap). Probable trpS,
                     tryptophanyl-tRNA synthetase (EC 6.1.1.2), equivalent to
                     Q49901|SYW_MYCLE|TRPS|ML0686|L308_C1_147 TRYPTOPHANYL-TRNA
                     SYNTHETASE from Mycobacterium leprae (343 aa), FASTA
                     scores: opt: 1859, E(): 4.8e-107, (83.75% identity in 339
                     aa overlap). Also similar to many e.g. Q9KZA7|TRPS2 from
                     Streptomyces coelicolor (339 aa), FASTA scores: opt: 1359,
                     E(): 2.6e-76, (60.3% identity in 335 aa overlap);
                     Q9EYY6|TRPS from Klebsiella aerogenes (334 aa), FASTA
                     scores: opt: 1077, E(): 5.5e-59, (52.15% identity in 328
                     aa overlap); P00954|SYW_ECOLI|TRPS|B3384 from Escherichia
                     coli strain K12 (334 aa), FASTA scores: opt: 1074, E():
                     8.3e-59, (51.85% identity in 328 aa overlap); etc.
                     Contains PS00178 Aminoacyl-transfer RNA synthetases
                     class-I signature. BELONGS TO CLASS-I AMINOACYL-TRNA
                     SYNTHETASE FAMILY. Protein product from Mb3369c detected
                     using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb3369c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P67591"
                     /db_xref="InterPro:IPR001412"
                     /db_xref="InterPro:IPR002305"
                     /db_xref="InterPro:IPR002306"
                     /db_xref="InterPro:IPR014729"
                     /db_xref="InterPro:IPR024109"
                     /db_xref="UniProtKB/Swiss-Prot:P67591"
BEGIN
        1 MSTPTGSRRI FSGVQPTSDS LHLGNALGAV AQWVGLQDDH DAFFCVVDLH AITIPQDPEA
       61 LRRRTLITAA QYLALGIDPG RATIFVQSQV PAHTQLAWVL GCFTGFGQAS RMTQFKDKSA
      121 RQGSEATTVG LFTYPVLQAA DVLAYDTELV PVGEDQRQHL ELARDVAQRF NSRFPGTLVV
      181 PDVLIPKMTA KIYDLQDPTS KMSKSAGTDA GLINLLDDPA LSAKKIRSAV TDSERDIRYD
      241 PDVKPGVSNL LNIQSAVTGT DIDVLVDGYA GHGYGDLKKD TAEAVVEFVN PIQARVDELT
      301 ADPAELEAVL AAGAQRAHDV ASKTVQRVYD RLGFLL
//