LOCUS SIU01992.1 405 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable
penicillin-binding protein dacb1 (d-alanyl-d-alanine carboxypeptidas
e) (dd-peptidase) (dd-carboxypeptidase) (pbp) (dd-transpeptidase)
(serine-type d-ala-d-ala carboxypeptidase) (d-amino acid
hydrolase) protein.
ACCESSION LT708304-3406
PROTEIN_ID SIU01992.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="dacB1"
/locus_tag="BQ2027_MB3363"
/note="Mb3363, dacB1, len: 405 aa. Equivalent to Rv3330,
len: 405 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 405 aa overlap). Probable dacB1,
D-alanyl-D-alanine carboxypeptidase (penicillin-binding
protein) (EC 3.4.16.4), equivalent to Mycobacterium leprae
proteins Q9CCM2|ML0691 PUTATIVE D-ALANYL-D-ALANINE
CARBOXYPEPTIDASE (411 aa), FASTA scores: opt: 2066, E():
2.5e-102, (77.15% identity in 416 aa overlap);
Q49917|L308_F1_36 (228 aa), FASTA scores: opt: 1241, E():
7.9e-59, (78.9% identity in 232 aa overlap) (note that
this protein corresponds to C-terminal part of the
putative protein encoded by Rv3330, aa 174-405); and
Q49921|PBPC (182 aa), FASTA scores: opt: 736, E():
3.7e-32, (73.95% identity in 169 aa overlap) (note that
this protein corresponds to N-terminal part of the
putative protein encoded by Rv3330, aa 1-158); note
L308_F1_36 (228 aa) and PBPC (182 aa) are two consecutive
Mycobacterium leprae ORFs . Also similar to others e.g.
Q9FC34|SC4G1.16c PUTATIVE D-ALANYL-D-ALANINE
CARBOXYPEPTIDASE from Streptomyces coelicolor (413 aa),
FASTA scores: opt: 572, E(): 3.4e-23, (33.75% identity in
382 aa overlap); P35150|DACB_BACSU PENICILLIN-BINDING
PROTEIN 5* PRECURSOR (D-ALANYL-D-ALANINE CARBOXYPEPTIDASE)
from Bacillus subtilis (382 aa), FASTA scores: opt: 422,
E(): 2.8e-15, (31.3% identity in 249 aa overlap);
Q9K8X5|DACB|BH2877 D-ALANYL-D-ALANINE CARBOXYPEPTIDASE
(PENICILLIN-BINDING PROTEIN) from Bacillus halodurans (395
aa), FASTA scores: opt: 421, E(): 3.2e-15, (31.95%
identity in 241 aa overlap); etc. Also similar to M.
tuberculosis Q10828|Rv2911|MTCY274.43 PROBABLE
PENICILLIN-BINDING PROTEIN (BELONGS TO PEPTIDASE FAMILY
S11; ALSO KNOWN AS THE D-ALANYL-D-ALANINE CARBOXYPEPTIDASE
1 FAMILY) (291 aa), FASTA scores: opt: 746, E(): 1.6e-32,
(47.0% identity in 266 aa overlap). Has hydrophobic
stretches at both N- and C-termini. Certainly
membrane-bound protein. BELONGS TO PEPTIDASE FAMILY S11;
ALSO KNOWN AS THE D-ALANYL-D-ALANINE CARBOXYPEPTIDASE 1
FAMILY. Protein product from Mb3363 detected using SWATH
mass spectrometry. Mb3363 found to be expressed during
exponential growth in Sauton's minimal media by
RNA-sequencing."
/db_xref="GOA:A0A1R3Y3V5"
/db_xref="InterPro:IPR001967"
/db_xref="InterPro:IPR012338"
/db_xref="InterPro:IPR018044"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y3V5"
BEGIN
1 MAFLRSVSCL AAAVFAVGTG IGLPTAAGEP NAAPAACPYK VSTPPAVDSS EVPAAGEPPL
61 PLVVPPTPVG GNALGGCGII TAPGSAPAPG DVSAEAWLVA DLDSGAVIAA RDPHGRHRPA
121 SVIKVLVAMA SINTLTLNKS VAGTADDAAV EGTKVGVNTG GTYTVNQLLH GLLMHSGNDA
181 AYALARQLGG MPAALEKINL LAAKLGGRDT RVATPSGLDG PGMSTSAYDI GLFYRYAWQN
241 PVFADIVATR TFDFPGHGDH PGYELENDNQ LLYNYPGALG GKTGYTDDAG QTFVGAANRD
301 GRRLMTVLLH GTRQPIPPWE QAAHLLDYGF NTPAGTQIGT LIEPDPSLMS TDRNPADRQR
361 VDPQAAARIS AADALPVRVG VAVIGALIVF GLIMVARAMN RRPQH
//