LOCUS SIU01972.1 427 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE THYMIDINE PHOSPHORYLASE DEOA (TDRPASE) (PYRIMIDINE PHOSPHORYLASE) protein. ACCESSION LT708304-3386 PROTEIN_ID SIU01972.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="deoA" /locus_tag="BQ2027_MB3343C" /note="Mb3343c, deoA, len: 427 aa. Equivalent to Rv3314c, len: 427 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 427 aa overlap). Probable deoA, thymidine phosporylase (EC 2.4.2.4), highly similar to many e.g. Q9AK36|DEOA from Streptomyces coelicolor (427 aa), FASTA scores: opt: 1668, E(): 3.2e-90, (62.35% identity in 425 aa overlap); Q9CFM5|PDP from Lactococcus lactis (subsp. lactis) (Streptococcus lactis) (430 aa), FASTA scores: opt: 1031, E(): 5.5e-53, (46.45% identity in 392 aa overlap); P19971|TYPH_HUMAN|ECGF1 from Homo sapiens (Human) (482 aa), FASTA scores: opt: 957, E(): 1.3e-48, (44.45% identity in 441 aa overlap); P07650|TYPH_ECOLI|DEOA|TPP|TTG|B4382 from Escherichia coli strain K12 (440 aa), FASTA scores: opt: 847, E(): 3.2e-42, (41.55% identity in 438 aa overlap); etc. Contains PS00647 Thymidine and pyrimidine-nucleoside phosphorylases signature. BELONGS TO THE THYMIDINE/PYRIMIDINE-NUCLEOSIDE PHOSPHORYLASES FAMILY. Protein product from Mb3343c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3343c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3S3" /db_xref="InterPro:IPR000053" /db_xref="InterPro:IPR000312" /db_xref="InterPro:IPR013102" /db_xref="InterPro:IPR017459" /db_xref="InterPro:IPR017872" /db_xref="InterPro:IPR018090" /db_xref="InterPro:IPR035902" /db_xref="InterPro:IPR036320" /db_xref="InterPro:IPR036566" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3S3" BEGIN 1 MTDFAFDAPT VIRTKRDGGR LSDAAIDWVV KAYTDGRVAD EQMSALLMAI VWRGMDRGEI 61 ARWTAAMLAS GARLDFTDLP LATVDKHSTG GVGDKITLPL VPVVAACGGA VPQASGRGLG 121 HTGGTLDKLE SITGFTANLS NQRVREQLCD VGAAIFAAGQ LAPADAKLYA LRDITGTVES 181 LPLIASSIMS KKLAEGAGAL VLDVKVGSGA FMRSPVQARE LAHTMVELGA AHGVPTRALL 241 TEMNCPLGRT VGNALEVAEA LEVLAGGGPP DVVELTLRLA GEMLELAGIH GRDPAQTLRD 301 GTAMDRFRWL VAAQGGDLSK PLPIGSHSET VTAGASGTMG DIDAMAVGLA AWRLGAGRSR 361 PGARVQHGAG VRIHRRPGEP VVVGEPLFTL YTNAPERFGA ARAELAGGWS IRDSPPQVRP 421 LIVDRIV //