LOCUS SIU01971.1 365 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ADENOSINE DEAMINASE ADD (ADENOSINE AMINOHYDROLASE) protein. ACCESSION LT708304-3385 PROTEIN_ID SIU01971.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="add" /locus_tag="BQ2027_MB3342C" /note="Mb3342c, add, len: 365 aa. Equivalent to Rv3313c, len: 365 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 365 aa overlap). Probable add, adenosine deaminase (EC 3.5.4.4), equivalent to Q9CCL9|ADD|ML0700 PUTATIVE ADENOSINE DEAMINASE from Mycobacterium leprae (362 aa), FASTA scores: opt: 2097, E(): 1.4e-127, (88.2% identity in 356 aa overlap) . Also similar to many e.g. Q9AK25|2SCK8.27 from Streptomyces coelicolor (396 aa), FASTA scores: opt: 1578, E(): 3.7e-94, (66.65% identity in 360 aa overlap); Q17747|C06G3.5 from Caenorhabditis elegans (349 aa), FASTA scores: opt: 435, E(): 1.1e-20, (29.6% identity in 348 aa overlap); P22333|ADD_ECOLI|B1623 from Escherichia coli strain K12 (333 aa), FASTA scores: opt: 380, E(): 3.7e-17, (29.4% identity in 340 aa overlap); etc. BELONGS TO THE ADENOSINE AND AMP DEAMINASES FAMILY. Protein product from Mb3342c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3342c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P63908" /db_xref="InterPro:IPR001365" /db_xref="InterPro:IPR006330" /db_xref="InterPro:IPR028893" /db_xref="InterPro:IPR032466" /db_xref="UniProtKB/Swiss-Prot:P63908" BEGIN 1 MTAAPTLQTI RLAPKALLHD HLDGGLRPAT VLDIAGQVGY DDLPATDVDA LASWFRTQSH 61 SGSLERYLEP FSHTVAVMQT PEALYRVAFE CAQDLAADSV VYAEVRFAPE LHISCGLSFD 121 DVVDTVLTGF AAGEKACAAD GQPITVRCLV TAMRHAAMSR EIAELAIRFR DKGVVGFDIA 181 GAEAGHPPTR HLDAFEYMRD HNARFTIHAG EAFGLPSIHE AIAFCGADRL GHGVRIVDDI 241 DVDADGGFQL GRLAAILRDK RIPLELCPSS NVQTGAVASI AEHPFDLLAR ARFRVTVNTD 301 NRLMSDTSMS LEMHRLVEAF GYGWSDLARF TVNAMKSAFI PFDQRLAIID EVIKPRFAAL 361 MGHSE //