LOCUS SIU01960.1 471 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 nad( p)h quinone reductase lpda protein. ACCESSION LT708304-3374 PROTEIN_ID SIU01960.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="lpdA" /locus_tag="BQ2027_MB3331C" /note="Mb3331c, lpdA, len: 471 aa. Equivalent to Rv3303c, len: 493 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 471 aa overlap). Probable lpdA, dihydrolipoamide dehydrogenase (EC 1.8.1.4), similar to other e.g. Q9EWV3|2SCK31.22c PUTATIVE OXIDOREDUCTASE from Streptomyces coelicolor (475 aa), FASTA scores: opt: 1420, E(): 2.4e-77, (54.9% identity in 471 aa overlap); Q9A7J2|CC1731 LIPOAMIDE DEHYDROGENASE (E3 COMPONENT,PYRUVATE DEHYDROGENASE COMPLEX) from Caulobacter crescentus (466 aa), FASTA scores: opt: 696, E(): 3.6e-34, (29.6% identity in 463 aa overlap); Q04829|LPD|DLDH_HALVO DIHYDROLIPOAMIDE DEHYDROGENASE from Halobacterium volcanii (Haloferax volcanii) (474 aa), FASTA scores: opt: 675, E(): 6.5e-33, (29.3% identity in 471 aa overlap); P50970|DLDH_ZYMMO|LPD DIHYDROLIPOAMIDE DEHYDROGENASE from Zymomonas mobilis, FASTA scores: opt: 658, E(): 6.6e-32, (30.4% identity in 464 aa overlap); etc. BELONGS TO THE PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASES CLASS-I. COFACTOR: FAD (BY SIMILARITY). REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, truncation due to a single base transversion (c-a) results in a shorter product compared to its homolog in Mycobacterium tuberculosis stain H37Rv (471 aa versus 493 aa). Protein product from Mb3331c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3331c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y3R8" /db_xref="InterPro:IPR001100" /db_xref="InterPro:IPR004099" /db_xref="InterPro:IPR016156" /db_xref="InterPro:IPR023753" /db_xref="InterPro:IPR036188" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3R8" BEGIN 1 MVTRIVILGG GPAGYEAALV AATSHPETAQ VTVIDCDGIG GAAVLDDCVP SKTFIASTGL 61 RTELRRAPHL GFHIDFDDAK ISLPQIHARV KTLAAAQSAD ITAQLLSMGV QVIAGRGELI 121 DSTPGLARHR IKATAADGST SEHEADVVLV ATGASPRILP SAQPDGERIL TWRQLYDLDA 181 LPDHLIVVGS GVTGAEFVDA YTELGVPVTV VASQDHVLPY EDADAALVLE ESFAERGVRL 241 FKNARAASVT RTGAGVLVTM TDGRTVEGSH ALMTIGSVPN TSGLGLERVG IQLGRGNYLT 301 VDRVSRTSAT GIYAAGDCTG LLPLASVAAM QGRIAMYHAL GEGVSPIRLR TVAATVFTRP 361 EIAAVGVPQS VIDAGSVAAR TIMLPLRTNA RAKMSEMRHG FVKIFCRRST GVVIGGVVVA 421 PIASELILPI AVAVQNRITV NELAQTLAVY PSLSGSITEA ARRLMAHDDL D //