LOCUS       SIU01960.1               471 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 nad(
            p)h quinone reductase lpda protein.
ACCESSION   LT708304-3374
PROTEIN_ID  SIU01960.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="lpdA"
                     /locus_tag="BQ2027_MB3331C"
                     /note="Mb3331c, lpdA, len: 471 aa. Equivalent to Rv3303c,
                     len: 493 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.6% identity in 471 aa overlap). Probable lpdA,
                     dihydrolipoamide dehydrogenase (EC 1.8.1.4), similar to
                     other e.g. Q9EWV3|2SCK31.22c PUTATIVE OXIDOREDUCTASE from
                     Streptomyces coelicolor (475 aa), FASTA scores: opt: 1420,
                     E(): 2.4e-77, (54.9% identity in 471 aa overlap);
                     Q9A7J2|CC1731 LIPOAMIDE DEHYDROGENASE (E3
                     COMPONENT,PYRUVATE DEHYDROGENASE COMPLEX) from Caulobacter
                     crescentus (466 aa), FASTA scores: opt: 696, E(): 3.6e-34,
                     (29.6% identity in 463 aa overlap); Q04829|LPD|DLDH_HALVO
                     DIHYDROLIPOAMIDE DEHYDROGENASE from Halobacterium volcanii
                     (Haloferax volcanii) (474 aa), FASTA scores: opt: 675,
                     E(): 6.5e-33, (29.3% identity in 471 aa overlap);
                     P50970|DLDH_ZYMMO|LPD DIHYDROLIPOAMIDE DEHYDROGENASE from
                     Zymomonas mobilis, FASTA scores: opt: 658, E(): 6.6e-32,
                     (30.4% identity in 464 aa overlap); etc. BELONGS TO THE
                     PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASES CLASS-I.
                     COFACTOR: FAD (BY SIMILARITY).
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis,
                     truncation due to a single base transversion (c-a) results
                     in a shorter product compared to its homolog in
                     Mycobacterium tuberculosis stain H37Rv (471 aa versus 493
                     aa). Protein product from Mb3331c detected using shotgun
                     mass spectrometry and SWATH mass spectrometry. Mb3331c
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y3R8"
                     /db_xref="InterPro:IPR001100"
                     /db_xref="InterPro:IPR004099"
                     /db_xref="InterPro:IPR016156"
                     /db_xref="InterPro:IPR023753"
                     /db_xref="InterPro:IPR036188"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y3R8"
BEGIN
        1 MVTRIVILGG GPAGYEAALV AATSHPETAQ VTVIDCDGIG GAAVLDDCVP SKTFIASTGL
       61 RTELRRAPHL GFHIDFDDAK ISLPQIHARV KTLAAAQSAD ITAQLLSMGV QVIAGRGELI
      121 DSTPGLARHR IKATAADGST SEHEADVVLV ATGASPRILP SAQPDGERIL TWRQLYDLDA
      181 LPDHLIVVGS GVTGAEFVDA YTELGVPVTV VASQDHVLPY EDADAALVLE ESFAERGVRL
      241 FKNARAASVT RTGAGVLVTM TDGRTVEGSH ALMTIGSVPN TSGLGLERVG IQLGRGNYLT
      301 VDRVSRTSAT GIYAAGDCTG LLPLASVAAM QGRIAMYHAL GEGVSPIRLR TVAATVFTRP
      361 EIAAVGVPQS VIDAGSVAAR TIMLPLRTNA RAKMSEMRHG FVKIFCRRST GVVIGGVVVA
      421 PIASELILPI AVAVQNRITV NELAQTLAVY PSLSGSITEA ARRLMAHDDL D
//