LOCUS SIU01957.1 305 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Pseudouridine synthase (EC , PA2043 type protein. ACCESSION LT708304-3371 PROTEIN_ID SIU01957.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3328C" /EC_number="4.2.1.70" /note="Mb3328c, -, len: 305 aa. Equivalent to Rv3300c, len: 305 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 305 aa overlap). Conserved hypothetical protein, similar to various proteins (notably pseudoridine synthase family proteins) e.g. Q9RJ76|SCI41.08 PUTATIVE RIBOSOMAL PSEUDOURIDINE SYNTHASE from Streptomyces coelicolor (324 aa), FASTA scores: opt: 876, E(): 4.5e-48, (52.1% identity in 313 aa overlap); Q9I272|PA2043 HYPOTHETICAL PROTEIN from Pseudomonas aeruginosa (300 aa), FASTA scores: opt: 676, E(): 1.8e-35, (42.55% identity in 268 aa overlap); Q9JZW8|NMB0867 YABO/YCEC/SFHB FAMILY PROTEIN from Neisseria meningitidis (serogroup B) (307 aa), FASTA scores: opt: 597, E(): 1.8e-30, (42.9% identity in 282 aa overlap); Q9JUY2|NMA1085 HYPOTHETICAL PROTEIN from Neisseria meningitidis (serogroup A) (307 aa), FASTA scores: opt: 597, E(): 1.8e-30, (42.9% identity in 282 aa overlap); Q12362|RIB2_YEAST|RIB2|YOL066C DRAP DEAMINASE (PSEUDOURIDINE SYNTHASE FAMILY PROTEIN) from Saccharomyces cerevisiae (Baker's yeast) (591 aa), FASTA scores: opt: 338, E(): 6.9e-14, (32.95% identity in 246 aa overlap); Q9RTS2|DR1684 PUTATIVE PSEUDOURIDINE SYNTHASE from Deinococcus radiodurans (321 aa), FASTA scores: opt: 319, E(): 6.5e-13, (32.75% identity in 235 aa overlap); etc. Also similar to Mycobacterium tuberculosis hypothetical protein Q10786|Y04P_MYCTU|MTCY48.25c|Rv1540|MT1592 (308 aa) (28.8% identity in 299 aa overlap). Mb3328c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3U6" /db_xref="InterPro:IPR006145" /db_xref="InterPro:IPR006224" /db_xref="InterPro:IPR020103" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3U6" BEGIN 1 MALRPEDRLL SVHDVLGPVR VRLLGGSVLA ELTARFGVAA RAKVLAGEVV DNDGAVVDSG 61 TVLPPGSVVH LYRDLPDEVP VPFDVPVLHQ DADIVVVDKP HFLATMPRGR HVAQTALVRL 121 RRELGLPELS PAHRLDRLTA GVLLFTTRRE VRGSYQTMFA RGLVRKTYLA RAPVAPGLAL 181 PRLVRSRIVK RRGHLQAVCE PGVPNAETLV ERIARDGLYR LTPTTGRTHQ LRVHMAALGI 241 PIMGDPLYPN VISVAAHDFS TPLQLLAQRI EFDDPLTGSH REFASTRTLT GATLPTWSAA 301 ADCRP //