LOCUS SIU01956.1 970 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ARYLSULFATASE ATSB (ARYL-SULFATE SULPHOHYDROLASE) (SULFATASE) protein. ACCESSION LT708304-3370 PROTEIN_ID SIU01956.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="atsB" /locus_tag="BQ2027_MB3327C" /note="Mb3327c, atsB, len: 970 aa. Equivalent to Rv3299c, len: 970 aa, from Mycobacterium tuberculosis strain H37Rv, (99.9% identity in 970 aa overlap). Probable atsB, arylsulfatase (EC 3.1.6.1), similar to P51691|ARS_PSEAE|ATSA|PA0183 (alias CAA88421|ATSA) from Pseudomonas aeruginosa (535 aa), FASTA scores: opt: 645, E(): 5.8e-31, (32.0% identity in 550 aa overlap); Q9L4Y2|ATSA from Klebsiella pneumoniae (577 aa), FASTA scores: opt: 504, E(): 1.7e-22, (26.3% identity in 566 aa overlap); and P20713|ATSA|ARS_KLEAE (precursor) from Klebsiella pneumoniae (464 aa), FASTA scores: opt: 502, E(): 1.8e-22, (26.85% identity in 451 aa overlap). Also similar to Mycobacterium tuberculosis proteins O06776|MTI376.13c|ATSD|Rv0663 (787 aa) (43.6% identity in 796 aa overlap) and P95059|MTCY210.30|ATSA|R0711 (787 aa) (38.4% identity in 797 aa overlap). Equivalent to AAK47741 from Mycobacterium tuberculosis strain CDC1551 (992 aa) but shorter 22 aa. Contains PS00523 Sulfatases signature 1 and PS01095 Chitinases family 18 active site signature. BELONGS TO THE SULFATASE FAMILY. Protein product from Mb3327c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3327c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y4G2" /db_xref="InterPro:IPR000917" /db_xref="InterPro:IPR009200" /db_xref="InterPro:IPR017850" /db_xref="InterPro:IPR024607" /db_xref="UniProtKB/TrEMBL:A0A1R3Y4G2" BEGIN 1 MMSEDNALVL VAGYQDLDSA RHDFQTLVDA AKDKSIPLQG AVLIGKDAEG SPVLVDTGNR 61 LGRRGAAWGA GVGLAIGLFS PALLASAALG AATGALAGTF AHHRIKTGLA DKIGQALAAG 121 RAVVIAVTEA QGRLEAGQAL ASSPMKSVAE LSRSTLRSLG AALREAMGKF NPDRTRLPLP 181 QRRFGGVVGR TMAESVGDWS IVPSPFPPDD APNVLIVLID DAGFGGPDTF GGAIRTPTLS 241 RLAQNGLIYN RFHVTAVCSP TRAALLTGRN HHRVGFGSVC EFPGPYPGYS AVRPRSCAAL 301 PRILRDNGYV TGAFGKWHLT PDNVQGAAGP FDNWPLGWGF DHFWGFPSGA AGQYDPIISQ 361 DNSVIGIPEG SGEDGRPYYF PDDLTDKAIE WLHTVRAQNA TKPWMLYYAT GATHAPHHVF 421 KEWADKYRGE FDDGWDVYRQ KTFERQKRLG IIPPDAELTE RPDLFPAWDS MSEAQKRLFA 481 RQMEVFAGFS ENADWNVGRL LDAIEDLGES DNTLVFYIWG DNGASMEGTN TGSFNEMTFL 541 NGLDLDAERQ LELIEQYGGI AALGDEFTAP HFASAWAHAS NTPLQWGKQM ASHLGGTRDP 601 LVVAWPARIR PDGRVRSQFT HCIDIAPTVL AAIGLPEPTH VDGFEQEPMD GTSFVRTFDD 661 AEAEDRHTVQ YFENFGSRAI YKDGWWACAR LDKAPWDLSP ETMRRFAPGT YDPDQDVWEL 721 YYLPDDFSQA KNLAAEHPDK VAELTQLWWQ EAERNRVLPL LGGLAVMFGD LPPLPTTARF 781 SFKGDVQNIQ RGMVPRICGR SYAIEARLHI PDGGAQGVIV ANADFMGGFA LWVDEQRHLH 841 HTYSFLGVET YRQVSSEPLP TGDVTVRMLF DSHQPVAASG GRVTLWADDR LIGEGELPQT 901 VPLAFTSYAG MDIGRDNGLV VDRGYEDKAP YAFTGTVTEV IFDLKPVHPE AARALHEHAS 961 VQAVGQGAAG //