LOCUS SIU01950.1 494 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PIPERIDEINE-6-CARBOXILIC ACID DEHYDROGENASE PCD (PIPERIDEINE- 6-CARBOXYLATE DEHYDROGENASE) protein. ACCESSION LT708304-3364 PROTEIN_ID SIU01950.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pcd" /locus_tag="BQ2027_MB3321" /note="Mb3321, pcd, len: 494 aa. Equivalent to Rv3293, len: 494 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 494 aa overlap). Probable pcd, piperideine-6-carboxylic acid dehydrogenase (EC 1.5.-.-), highly similar to others e.g. O85725|PCD SEMIALDEHYDE DEHYDROGENASE from Streptomyces clavuligerus (512 aa), FASTA scores: opt: 2214, E(): 6.7e-121, (68.75% identity in 496 aa overlap) (see first citation below); Q9I4U7|PA1027 PROBABLE ALDEHYDE DEHYDROGENASE from Pseudomonas aeruginosa (529 aa), FASTA scores: opt: 1984, E(): 1.4e-107, (64.5% identity in 493 aa overlap); BAB49892|MLL2867 ALDEHYDE DEHYDROGENASE from Rhizobium loti (Mesorhizobium loti) (504 aa), FASTA scores: opt: 1964, E(): 2e-106, (62.8% identity in 476 aa overlap); Q9A8Y1|CC1216 ALDEHYDE DEHYDROGENASE from Caulobacter crescentus (507 aa), FASTA scores: opt: 1909, E(): 3.1e-103, (59.95% identity in 497 aa overlap); O54199|PCD PIPERIDEINE-6-CARBOXILIC ACID DEHYDROGENASE from Streptomyces clavuligerus (496 aa), FASTA scores: opt: 1748, E(): 6.4e-94, (60.6% identity in 467 aa overlap); and Q9F1U8|PCD PIPERIDEINE-6-CARBOXYLATE DEHYDROGENASE from 'Flavobacterium' lutescens (510 aa), FASTA scores: opt: 1656, E(): 1.4e-88, (54.05% identity in 481 aa overlap) (see second citation below); etc. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site. Note that ORF Rv3290c seems to encoded the putative lat enzyme. Previously known as aldB. Protein product from Mb3321 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3321 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3Q7" /db_xref="InterPro:IPR015590" /db_xref="InterPro:IPR016161" /db_xref="InterPro:IPR016162" /db_xref="InterPro:IPR016163" /db_xref="InterPro:IPR029510" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3Q7" BEGIN 1 MLEACQAIGV TAALGEPGEH SLPASTPITG DVLFSIAPTT PEQADHAIAA AAATFTAWRS 61 TPAPVRGALV ARLGELLTAH QQDLATLVTV EVGKITAEAR GEVQEMIDVC QFSVGLSRQL 121 YGRTIASERA GHRLLETWHP LGVVGVITAF NFPVAVWAWN TAVALVCGDT VVWKPSELTP 181 LTALACQALL SRAAADVGAP AAVGGLLLGG AERGAQLVDD PRVALLSATG SVRMGQQVGP 241 RVARRFGRVL LELGGNNAAI VAPSADLELA VRCIVFAAAG TAGQRCTSLR RLIVHRSVAD 301 DVVARVVGAY RQLAIGDPSA PDTLVGPLIH EAAYRDMVAA LERARTDGGE VIGGDRREVG 361 SPGAYYVAPA VVRMPSQTAI VATETFAPIL YVLTYDDLDE AIALNNAVPQ GLSSSIFTTD 421 LREAEHFLDQ SDCGIANVNI GTSGAEIGGA FGGEKQTGGG RESGSDAWKA YMRRATNTVN 481 YSSELPLAQG VKFG //