LOCUS       SIU01943.1               261 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 alternative
            rna polymerase sigma factor sigf protein.
ACCESSION   LT708304-3357
PROTEIN_ID  SIU01943.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="sigF"
                     /locus_tag="BQ2027_MB3314C"
                     /note="Mb3314c, sigF, len: 261 aa. Equivalent to Rv3286c,
                     len: 261 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 261 aa overlap). Probable sigF, stress
                     response/stationary phase RNA polymerase sigma factor (see
                     citations below), similar to several Streptomyces RNA
                     polymerase sigma factors e.g. Q9RPC8|SIGH from
                     Streptomyces coelicolor A3(2) (354 aa), FASTA scores: opt:
                     869, E(): 1.1e-45, (51.15% identity in 258 aa overlap);
                     Q9RIT0|SIG1 from Streptomyces coelicolor (361 aa), FASTA
                     scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa
                     overlap); Q9ADM4|2SC10A7.38c from Streptomyces coelicolor
                     (318 aa), FASTA scores: opt: 776, E(): 4.6e-40, (48.75%
                     identity in 240 aa overlap);
                     P37971|RPOF_STRCO|SIGF|RPOX|2SCD60.01c from Streptomyces
                     coelicolor (287 aa), FASTA scores: opt: 717, E(): 1.6e-36,
                     (44.5% identity in 245 aa overlap);
                     P37970|RPOF_STRAU|SIGF|RPOX from Streptomyces aureofaciens
                     (297 aa); etc. Contains possible helix-turn-helix motif at
                     aa 229-250 (+7.38 SD). SIMILAR TO THE SIGMA-70 FACTOR
                     FAMILY. Seems expressed in stationary phase and under
                     stress conditions in vitro (see citations below). Protein
                     product from Mb3314c detected using SWATH mass
                     spectrometry. Mb3314c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y3Q3"
                     /db_xref="InterPro:IPR000943"
                     /db_xref="InterPro:IPR007624"
                     /db_xref="InterPro:IPR007627"
                     /db_xref="InterPro:IPR007630"
                     /db_xref="InterPro:IPR013324"
                     /db_xref="InterPro:IPR013325"
                     /db_xref="InterPro:IPR014284"
                     /db_xref="InterPro:IPR014322"
                     /db_xref="InterPro:IPR036388"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y3Q3"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MTARAAGGSA SRANEYADVP EMFRELVGLP AGSPEFQRHR DKIVQRCLPL ADHIARRFEG
       61 RGEPRDDLIQ VARVGLVNAA VRFDVKTGSD FVSFAVPTIM GEVRRHFRDN SWSVKVPRRL
      121 KELHLRLGTA TADLSQRLGR APSASELAAE LGMDRAEVIE GLLAGSSYHT LSIDSGGGSD
      181 DDARAITDTL GDVDAGLDQI ENREVLRPLL EALPERERTV LVLRFFDSMT QTQIAERVGI
      241 SQMHVSRLLA KSLARLRDQL E
//