LOCUS SIU01943.1 261 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 alternative rna polymerase sigma factor sigf protein. ACCESSION LT708304-3357 PROTEIN_ID SIU01943.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="sigF" /locus_tag="BQ2027_MB3314C" /note="Mb3314c, sigF, len: 261 aa. Equivalent to Rv3286c, len: 261 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 261 aa overlap). Probable sigF, stress response/stationary phase RNA polymerase sigma factor (see citations below), similar to several Streptomyces RNA polymerase sigma factors e.g. Q9RPC8|SIGH from Streptomyces coelicolor A3(2) (354 aa), FASTA scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa overlap); Q9RIT0|SIG1 from Streptomyces coelicolor (361 aa), FASTA scores: opt: 869, E(): 1.1e-45, (51.15% identity in 258 aa overlap); Q9ADM4|2SC10A7.38c from Streptomyces coelicolor (318 aa), FASTA scores: opt: 776, E(): 4.6e-40, (48.75% identity in 240 aa overlap); P37971|RPOF_STRCO|SIGF|RPOX|2SCD60.01c from Streptomyces coelicolor (287 aa), FASTA scores: opt: 717, E(): 1.6e-36, (44.5% identity in 245 aa overlap); P37970|RPOF_STRAU|SIGF|RPOX from Streptomyces aureofaciens (297 aa); etc. Contains possible helix-turn-helix motif at aa 229-250 (+7.38 SD). SIMILAR TO THE SIGMA-70 FACTOR FAMILY. Seems expressed in stationary phase and under stress conditions in vitro (see citations below). Protein product from Mb3314c detected using SWATH mass spectrometry. Mb3314c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3Q3" /db_xref="InterPro:IPR000943" /db_xref="InterPro:IPR007624" /db_xref="InterPro:IPR007627" /db_xref="InterPro:IPR007630" /db_xref="InterPro:IPR013324" /db_xref="InterPro:IPR013325" /db_xref="InterPro:IPR014284" /db_xref="InterPro:IPR014322" /db_xref="InterPro:IPR036388" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3Q3" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTARAAGGSA SRANEYADVP EMFRELVGLP AGSPEFQRHR DKIVQRCLPL ADHIARRFEG 61 RGEPRDDLIQ VARVGLVNAA VRFDVKTGSD FVSFAVPTIM GEVRRHFRDN SWSVKVPRRL 121 KELHLRLGTA TADLSQRLGR APSASELAAE LGMDRAEVIE GLLAGSSYHT LSIDSGGGSD 181 DDARAITDTL GDVDAGLDQI ENREVLRPLL EALPERERTV LVLRFFDSMT QTQIAERVGI 241 SQMHVSRLLA KSLARLRDQL E //