LOCUS SIU01940.1 297 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE THIOSULFATE SULFURTRANSFERASE SSEA (RHODANESE) (THIOSULFATE CYANIDE TRANSSULFURASE) (THIOSULFATE THIOTRANSFERASE) protein. ACCESSION LT708304-3354 PROTEIN_ID SIU01940.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="sseA" /locus_tag="BQ2027_MB3311" /note="Mb3311, sseA, len: 297 aa. Equivalent to Rv3283, len: 297 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 297 aa overlap). Probable sseA, thiosulfate sulfurtransferase (EC 2.8.1.1), equivalent P46700|THT2_MYCLE|SSEA|ML0728|B1308_C1_127 PUTATIVE THIOSULFATE SULFURTRANSFERASE SSEA from Mycobacterium leprae (296 aa), FASTA scores: opt: 1742, E(): 5.5e-108, (83.45% identity in 296 aa overlap). Also highly similar to others e.g. Q9RXT9|DR0217 from Deinococcus radiodurans (286 aa), FASTA scores: opt: 1057, E(): 1.2e-62, (53.86% identity in 273 aa overlap); P16385|THTR_SACER|CYSA from Saccharopolyspora erythraea (Streptomyces erythraeus) (281 aa), FASTA scores: opt: 1006, E(): 2.7e-59, (51.25% identity in 277 aa overlap); P71121|THTR_CORGL from Corynebacterium glutamicum (Brevibacterium flavum) (225 aa), FASTA scores: opt: 897, E(): 3.6e-52, (59.05% identity in 215 aa overlap); etc. Also highly similar to O05793|CYSA1|CYSA|Rv3117|MT3199|MTCY164.27|CYSA2|RV0815c|M T0837|MTV043.07c|THTR_MYCTU PUTATIVE THIOSULFATE SULFURTRANSFERASE (EC 2.8.1.1) from Mycobacterium tuberculosis (277 aa), FASTA scores: opt: 955, E(): 6.3e-56, (50.2% identity in 271 aa overlap); and Q50036|THTR_MYCLE|CYSA|CYSA3|ML2198 PUTATIVE THIOSULFATE SULFURTRANSFERASE from Mycobacterium leprae (277 aa), FASTA scores: opt: 931, E(): 2.5e-54, (48.9% identity in 276 aa overlap). Shows some similarity to MTCY339.19c (30.3% identity in 254 aa overlap). Contains PS00683 Rhodanese C-terminal signature. BELONGS TO THE RHODANESE FAMILY. Protein product from Mb3311 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3311 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:Q7TWT6" /db_xref="InterPro:IPR001307" /db_xref="InterPro:IPR001763" /db_xref="InterPro:IPR036873" /db_xref="UniProtKB/Swiss-Prot:Q7TWT6" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MPLPADPSPT LSAYAHPERL VTADWLSAHM GAPGLAIVES DEDVLLYDVG HIPGAVKIDW 61 HTDLNDPRVR DYINGEQFAE LMDRKGIARD DTVVIYGDKS NWWAAYALWV FTLFGHADVR 121 LLNGGRDLWL AERRETTLDV PTKTCTGYPV VQRNDAPIRA FRDDVLAILD AQPLIDVRSP 181 EEYTGKRTHM PDYPEEGALR AGHIPTAVHI PWGKAADESG RFRSREELER LYDFINPDDQ 241 TVVYCRIGER SSHTWFVLTH LLGKADVRNY DGSWTEWGNA VRVPIVAGEE PGVVPVV //