LOCUS SIU01938.1 156 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable bifunctional protein acetyl-/propionyl-coenzyme a carboxylase (epsilon chain) acce5 protein. ACCESSION LT708304-3352 PROTEIN_ID SIU01938.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="acce5" /locus_tag="BQ2027_MB3309" /note="Mb3309, -, len: 156 aa. Similar to Rv3281, len: 177 aa, from Mycobacterium tuberculosis strain H37Rv, (88.1% identity in 177 aa overlap). Conserved hypothetical protein, equivalent (but longer 14 aa and with a gap between aa 82-102) to AAK47723|MT3380 from Mycobacterium tuberculosis strain CDC1551 (142 aa), FASTA scores: opt: 830, E(): 3.1e-40, (86.5% identity in 163 aa overlap). C-terminus highly similar to Q49671|B1308_C3_211|ML0730 from Mycobacterium leprae (84 aa), FASTA scores: opt: 393, E(): 7.6e-16, (68.95% identity in 87 aa overlap). REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, an in-frame deletion of 63 bp leads to a shorter product compared to its homolog in Mycobacterium tuberculosis strain H37Rv (156 aa versus 177 aa). Protein product from Mb3309 detected using shotgun mass spectrometry. Mb3309 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="InterPro:IPR032716" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3Y2" BEGIN 1 MGTCPCESSE RNEPVSRVSG TNEVSDGNET NNPAEVSDGN ETNNPAEVSD GNETNNPAPV 61 SRVSGTNEVS DGNETNNPAP VTEKPLHPHE PHIEILRGQP TDQELAALIA VLGSISGSTP 121 PAQPEPTRWG LPVDQLRYPV FSWQRITLQE MTHMRR //