LOCUS SIU01935.1 172 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED TRANSMEMBRANE PROTEIN protein. ACCESSION LT708304-3349 PROTEIN_ID SIU01935.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3306C" /note="Mb3306c, -, len: 172 aa. Equivalent to Rv3278c, len: 172 aa, from Mycobacterium tuberculosis strain H37Rv, (99.4% identity in 172 aa overlap). Probable conserved transmembrane protein, equivalent to Q9CCL2|ML0733 PUTATIVE MEMBRANE PROTEIN from Mycobacterium leprae (172 aa), FASTA scores: opt: 1024, E(): 6e-61, (83.15% identity in 172 aa overlap); and Q49672|B1308_F2_67 HYPOTHETICAL PROTEIN from Mycobacterium leprae (181 aa), FASTA scores: opt: 1024, E(): 6.3e-61, (83.15% identity in 172 aa overlap) (this is certainly the same putative protein but with N-terminus longer). Also some similarity to other hypothetical proteins (generally membrane proteins) e.g. O26822|MTH726 HYPOTHETICAL PROTEIN from Methanobacterium thermoautotrophicum (204 aa), FASTA scores: opt: 147, E(): 0.0079, (24.6% identity in 187 aa overlap); Q9X8H4|SCE9.01 HYPOTHETICAL 47.7 KDA PROTEIN (FRAGMENT) from Streptomyces coelicolor (436 aa), FASTA scores: opt: 151, E(): 0.0079, (28.1% identity in 153 aa overlap). Protein product from Mb3306c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3306c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y5H1" /db_xref="InterPro:IPR005182" /db_xref="UniProtKB/TrEMBL:A0A1R3Y5H1" BEGIN 1 MSYPENVLAA GEQVVLHRHP HWNRLIWPVV VLVLLTGLAA FGSGFVNSTP WQQIAKNVIH 61 AVIWGIWLVI VGWLTLWPFL SWLTTHFVVT NRRVMFRHGV LTRSGIDIPL ARINSVEFRD 121 RIFERIFRTG TLIIESASQD PLEFYNIPRL REVHALLYHK VFDTLGSDES PS //