LOCUS SIU01933.1 429 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE ATPASE SUBUNIT
PURK (AIR CARBOXYLASE) (AIRC) protein.
ACCESSION LT708304-3347
PROTEIN_ID SIU01933.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="purK"
/locus_tag="BQ2027_MB3304C"
/note="Mb3304c, purK, len: 429 aa. Equivalent to Rv3276c,
len: 429 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 429 aa overlap). Probable purK,
phosphoribosylaminoimidazole carboxylase ATPase subunit
(EC 4.1.1.21), equivalent to
P46701|PURK_MYCLE|ML0735|B1308_F1_32
PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE ATPASE SUBUNIT
from Mycobacterium leprae (439 aa), FASTA scores: opt:
2168, E(): 2.3e-123, (76.15% identity in 444 aa overlap).
Also similar to others e.g. Q44678|PURK_CORAM from
Corynebacterium ammoniagenes (Brevibacterium ammoniagenes)
(413 aa), FASTA scores: opt: 1179, E(): 9.1e-64, (48.35%
identity in 389 aa overlap); Q9KZ85|PURK from Streptomyces
coelicolor (368 aa), FASTA scores: opt: 1150, E():
4.7e-62, (55.35% identity in 345 aa overlap);
Q54975|PURK_SYNP7 from Synechococcus sp. strain PCC 7942
(Anacystis nidulans R2) (395 aa), FASTA scores: opt: 772,
E(): 3e-39, (38.1% identity in 383 aa overlap); etc.
BELONGS TO THE PURK / PURT FAMILY. Protein product from
Mb3304c detected using shotgun mass spectrometry and SWATH
mass spectrometry. Mb3304c found to be expressed during
exponential growth in Sauton's minimal media by
RNA-sequencing."
/db_xref="GOA:P65899"
/db_xref="InterPro:IPR003135"
/db_xref="InterPro:IPR005875"
/db_xref="InterPro:IPR011054"
/db_xref="InterPro:IPR011761"
/db_xref="InterPro:IPR013815"
/db_xref="InterPro:IPR016185"
/db_xref="InterPro:IPR040686"
/db_xref="UniProtKB/Swiss-Prot:P65899"
BEGIN
1 MMAVASSRTP AVTSFIAPLV AMVGGGQLAR MTHQAAIALG QNLRVLVTSA DDPAAQVTPN
61 VVIGSHTDLA ALRRVAAGAD VLTFDHEHVP NELLEKLVAD GVNVAPSPQA LVHAQDKLVM
121 RQRLAAAGVA VPRYAGIKDP DEIDVFAARV DAPIVVKAVR GGYDGRGVRM ARDVADARDF
181 ARECLADGVA VLVEERVDLR RELSALVARS PFGQGAAWPV VQTVQRDGTC VLVIAPAPAL
241 PDDLATAAQR LALQLADELG VVGVLAVELF ETTDGALLVN ELAMRPHNSG HWTIDGARTS
301 QFEQHLRAVL DYPLGDSDAV VPVTVMANVL GAAQPPAMSV DERLHHLFAR MPDARVHLYG
361 KAERPGRKVG HINFLGSDVA QLCERAELAA HWLSHGRWTD GWDPHRASDD AVGVPPACGG
421 RSDEEERRL
//