LOCUS SIU01932.1 174 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT PURE (AIR CARBOXYLASE) (AIRC) protein. ACCESSION LT708304-3346 PROTEIN_ID SIU01932.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="purE" /locus_tag="BQ2027_MB3303C" /note="Mb3303c, purE, len: 174 aa. Equivalent to Rv3275c, len: 174 aa, from Mycobacterium tuberculosis strain H37Rv, (99.4% identity in 174 aa overlap). Probable purE, phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21), equivalent to P46702|PUR6_MYCLE|PURE|ML0736|B1308_F3_98 from Mycobacterium leprae (171 aa), FASTA scores: opt: 878, E(): 1.5e-43, (81.55% identity in 168 aa overlap). Also similar to others e.g. Q9AXD0|AIRC from Nicotiana tabacum (Common tobacco) (623 aa), FASTA scores: opt: 712, E(): 1.4e-33, (69.35% identity in 160 aa overlap) (similarity in C-terminal part for this one); Q44679|PUR6_CORAM from Corynebacterium ammoniagenes (Brevibacterium ammoniagenes) (177 aa), FASTA scores: opt: 651, E(): 1.5e-30, (68.25% identity in 148 aa overlap); Q55498|PUR6_SYNY3|PURE|SLL0901 from Synechocystis sp. strain PCC 6803 (176 aa), FASTA scores: opt: 639, E(): 7.1e-30, (60.5% identity in 167 aa overlap); etc. Protein product from Mb3303c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3303c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y3M8" /db_xref="InterPro:IPR000031" /db_xref="InterPro:IPR024694" /db_xref="InterPro:IPR033747" /db_xref="InterPro:IPR035893" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3M8" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTPAGERPRV GVIMGSDSDW PVMADAAAAL AEFDIPAEVR VVSAHRTPEA MFSYARGAAE 61 RGLEVIIAGA GGAAHLPGMV AAATPLPVIG VPVPLGRLDG LDSLLSIVQM PAGVPVATVS 121 IGGARNAGLL AVRMLGAANP QLRARIVAFQ DRLADVVAAK DAELQRLAGK LTRD //