LOCUS SIU01904.1 214 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 thymidylate kinase tmk (dtmp kinase) (thymidylic acid kinase) (tmpk) protein. ACCESSION LT708304-3318 PROTEIN_ID SIU01904.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="tmk" /locus_tag="BQ2027_MB3275C" /note="Mb3275c, tmk, len: 214 aa. Equivalent to Rv3247c, len: 214 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 214 aa overlap). Probable tmk, thymidylate kinase (EC 2.7.4.9), equivalent to Q9CCJ3|TMK|ML0772 PUTATIVE THYMIDYLATE KINASE from Mycobacterium leprae (210 aa), FASTA scores: opt: 1023, E(): 4.8e-57, (77.3% identity in 207 aa overlap). Also similar to other thymidylate kinases e.g. Q9RQJ9|KTHY_CAUCR|TMK|CC1824 from Caulobacter crescentus (208 aa), FASTA scores: opt: 179, E(): 0.0003, (31.3% identity in 214 aa overlap); Q9V1E9|KTHY_PYRAB|TMK|PAB0319 from Pyrococcus abyssi (205 aa), FASTA scores: opt: 176, E(): 0.00045, (29.1% identity in 189 aa overlap); etc. BELONGS TO THE THYMIDYLATE KINASE FAMILY. Protein product from Mb3275c detected using SWATH mass spectrometry. Mb3275c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3J9" /db_xref="InterPro:IPR018094" /db_xref="InterPro:IPR018095" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR039430" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3J9" BEGIN 1 MLIAIEGVDG AGKRTLVEKL SGAFRAAGRS VATLAFPRYG QSVAADIAAE ALHGEHGDLA 61 SSVYAMATLF ALDRAGAVHT IQGLCRGYDV VILDRYVASN AAYSAARLHE NAAGKAAAWV 121 QRIEFARLGL PKPDWQVLLA VSAELAGERS RGRAQRDPGR ARDNYERDAE LQQRTGAVYA 181 ELAAQGWGGR WLVVGADVDP GRLAATLAPP DVPS //