LOCUS SIU01891.1 469 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 possible triacylglycerol synthase (diacylglycerol acyltransferase) protein. ACCESSION LT708304-3305 PROTEIN_ID SIU01891.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="tgs3" /locus_tag="BQ2027_MB3262C" /note="Mb3262c, -, len: 469 aa. Equivalent to Rv3234c and Rv3233c, len: 271 aa and 196 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 266 aa overlap and 100.0% identity in 196 aa overlap). Rv3234c: Hypothetical protein, similar to C-terminus of Mycobacterium tuberculosis hypothetical proteins e.g. P71694|Rv1425|MTCY21B4.43|MTCY493.29c (459 aa), FASTA scores: opt: 498, E(): 5.2e-24, (36.8% identity in 261 aa overlap); MTCY03A2.28; MTCY31.23; MTCY493_29; MTCY28_26; MTV013_8; MTY13E12_33; etc. Also similar to Q9X7A8|MLCB1610.05|ML1244 CONSERVED MEMBRANE PROTEIN from Mycobacterium leprae (491 aa), FASTA scores: opt: 309, E(): 4.3e-12, (33.35% identity in 189 aa overlap). Rv3233c: Hypothetical protein, similar to C-terminus of Q9RIU8|SCM11.13c HYPOTHETICAL 47.1 KDA PROTEIN from Streptomyces coelicolor (446 aa), FASTA scores: opt: 308, E(): 1.2e-12, (32.0% identity in 200 aa overlap); and several hypothetical Mycobacterium tuberculosis proteins e.g. O06343|YY80_MYCTU|Rv3480c|MTCY13E12.33c (497 aa), FASTA scores: opt: 248, E(): 9.8e-09, (27.5% identity in 200 aa overlap); MTCY28_26; MTCY493_29; MTCY31_25; MTCY31_25. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis H37Rv, Rv3234c and Rv3233c exist as 2 genes. In Mycobacterium bovis, a single base insertion (*-g) leads to a single product that is more similar to Rv3234c. Protein product from Mb3262c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3262c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3I4" /db_xref="InterPro:IPR004255" /db_xref="InterPro:IPR009721" /db_xref="InterPro:IPR014292" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3I4" BEGIN 1 MVTRLSASDA SFYQLENTAT PMYVGLLLIL RRPRAGLSYE ALLETVEQRL PQIPRYRQKV 61 QEVKLGLARP VWIDDRDFDI TYHVRRSALP SPGSDEQLHE LIARLAARPL DKSRPLWEMY 121 LVEGLEKNRI ALYTKSHQAL INGVTALAIG HVIADRTRRP PAFPEDIWVP ERDPGTTRLL 181 LRAVGDWLVR PGAQLQAVGS AVAGLVTNSG QLVETGRKVL DIARTVARGT APSSPLNATV 241 SRNRRFTVAR ASLDDYRTVR ARYDCDVHDV VLTVIAGALG NWLMSRGEAV APTATVRAMA 301 PLSVYADDQL DSTGPGQAIS QVTPFLVDLP VGEGNAVVRL SQIAHATESN PTAASLVDAR 361 TIVTLSGLAP ATLHAMGVRV ATSFSARLFN LLITNAPGTQ SQMYIAGTKL LETYSVPPLL 421 HNQALAISVT SYNGMLYFGI NADRDAMSDV DLLPGLLSQA LDELLEASR //