LOCUS SIU01869.1 203 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
PHOSPHOGLYCERATE MUTASE GPM2 (PHOSPHOGLYCEROMUTASE) (PGAM)
(BPG-DEPENDENT PGAM) protein.
ACCESSION LT708304-3283
PROTEIN_ID SIU01869.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="gpm2"
/locus_tag="BQ2027_MB3240"
/note="Mb3240, gpm2, len: 203 aa. Equivalent to Rv3214,
len: 203 aa, from Mycobacterium tuberculosis strain H37Rv,
(100.0% identity in 203 aa overlap). Possible gpm2,
phosphoglycerate mutase (EC 5.4.2.1), similar to many
mutases especially phosphoglycerate mutases e.g.
Q9F3H5|2SCC13.14c PUTATIVE MUTASE from Streptomyces
coelicolor (198 aa), FASTA scores: opt: 487, E(): 4.4e-25,
(42.25% identity in 194 aa overlap); BAB49378|MLL2186
PROBABLE PHOSPHOGLYCERATE MUTASE from Rhizobium loti
(Mesorhizobium loti) (193 aa), FASTA scores: opt: 423,
E(): 7e-21, (41.2% identity in 182 aa overlap);
Q9RKV8|SC9G1.08c PUTATIVE PHOSPHATASE from Streptomyces
coelicolor (199 aa), FASTA scores: opt: 419, E(): 1.3e-20,
(41.1% identity in 185 aa overlap); Q9RDL0|SCC123.14c
PUTATIVE PHOSPHOGLYCERATE MUTASE from Streptomyces
coelicolor (223 aa), FASTA scores: opt: 240, E(): 8.8e-09,
(36.9% identity in 168 aa overlap); Q9X194|TM1374
PHOSPHOGLYCERATE MUTASE from Thermotoga maritima (201 aa),
FASTA scores: opt: 218, E(): 2.3e-07, (33.15% identity in
202 aa overlap); etc. But N-terminus also similar to
Q9CCH5|ENTC|ML0808 PUTATIVE ISOCHORISMATE SYNTHASE from
Mycobacterium leprae (577 aa), FASTA scores: opt: 346,
E(): 2.1e-15, (55.05% identity in 109 aa overlap).
N-terminus shows also some similarity with other M.
tuberculosis proteins e.g. MTCY427.09c; MTCY20G9.15;
MTCY428.28. Equivalent to AAK47652 from Mycobacterium
tuberculosis strain CDC1551 (228 aa) but shorter 25 aa.
Note that previously known as entD. Protein product from
Mb3240 detected using shotgun mass spectrometry. Mb3240
found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing (TPM > 20)."
/db_xref="InterPro:IPR013078"
/db_xref="InterPro:IPR029033"
/db_xref="UniProtKB/TrEMBL:A0A1R3Y3F8"
BEGIN
1 MGVRNHRLLL LRHGETAWST LGRHTGGTEV ELTDTGRTQA ELAGQLLGEL ELDDPIVICS
61 PRRRTLDTAK LAGLTVNEVT GLLAEWDYGS YEGLTTPQIR ESEPDWLVWT HGCPAGESVA
121 QVNDRADSAV ALALEHMSSR DVLFVSHGHF SRAVITRWVQ LPLAEGSRFA MPTASIGICG
181 FEHGVRQLAV LGLTGHPQPI AAG
//