LOCUS SIU01853.1 355 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE TRANSMEMBRANE CATION TRANSPORTER protein. ACCESSION LT708304-3267 PROTEIN_ID SIU01853.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3225C" /note="Mb3225c, -, len: 355 aa. Equivalent to Rv3200c, len: 355 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 355 aa overlap). Possible transmembrane cation transporter, similar to many transmembrane proteins and putative potassium channels e.g. Q9XA52|SCGD3.27C PUTATIVE MEMBRANE PROTEIN from Streptomyces coelicolor (365 aa), FASTA scores: opt: 1022, E(): 2.6e-53, (49.85% identity in 325 aa overlap); Q9RRZ3|DR2336 PUTATIVE POTASSIUM CHANNEL from Deinococcus radiodurans (320 aa), FASTA scores: opt: 436, E(): 1e-18, (30.9% identity in 304 aa overlap); O28600|AF1673 PUTATIVE POTASSIUM CHANNEL from Archaeoglobus fulgidus (314 aa), FASTA scores: opt: 363, E(): 2.1e-14, (27.2% identity in 309 aa overlap); Q57604|Y13B_METJAMJ0138.1|MJ0138.1 PUTATIVE POTASSIUM CHANNEL from Methanococcus jannaschii (333 aa), FASTA scores: opt: 356, E(): 5.7e-14, (26.0% identity in 281 aa overlap); P73132|SLL0993 POTASSIUM CHANNEL from Synechocystis sp. strain PCC 6803 (365 aa), FASTA scores: opt: 330, E(): 2.1e-12, (27.8% identity in 324 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Protein product from Mb3225c detected using shotgun mass spectrometry. Mb3225c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3F6" /db_xref="InterPro:IPR003148" /db_xref="InterPro:IPR013099" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3F6" BEGIN 1 MAGSWRRLRG LDEKLTAQPG YALVGVLRIP QRRASPARVI SRRVVVAVVA LLLTAGIVYV 61 DRDGYLDAQG DRLTFLDCLY YAAVTLSTTG YGDITPISEF ARAINIFVIT PLRIAFLILL 121 VGTTLEVLTE TSRQAYKIQR WRSRVRNHTV VIGYGTKGKT AVAAMVSDEL VPGEIVVVDT 181 DSGVLERAAA AGLVTVHGDA TKSDVLRLAG TQHASSIIVA TSRDDTAVLV TLTAREIAPK 241 AKIVASIREA ENQHLLRQSG ADTVVVSSET AGRLLGIATT TPSVVEMIED LLTPEAGLAV 301 AEREVEQAEV GGSPRHLRDI VLGVVRDGQL LRIGAPEVDA IEASDRLLYI RQVGR //