LOCUS SIU01844.1 340 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE CONSERVED SECRETED PROTEIN protein. ACCESSION LT708304-3258 PROTEIN_ID SIU01844.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB3216C" /note="Mb3216c, -, len: 340 aa. Equivalent to Rv3194c, len: 340 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 340 aa overlap). Possible conserved secreted protein (N-terminal stretch hydrophobic), equivalent to Q9CCM7|ML0643 PUTATIVE SECRETED PROTEIN from Mycobacterium leprae (340 aa), FASTA scores: opt: 1822, E(): 1.6e-102, (80.3% identity in 340 aa overlap). Also similar to other proteins e.g. Q9FCI6|2SC3B6.26 PUTATIVE SECRETED PROTEIN from Streptomyces coelicolor (364 aa), FASTA scores: opt: 430, E(): 1.1e-18, (40.95% identity in 359 aa overlap); Q9S3Y5|SDRC SDRC PROTEIN from Streptomyces coelicolor (241 aa), FASTA scores: opt: 396, E(): 8.9e-17, (35.2% identity in 318 aa overlap) (similarity in part for this one); O34470|YLBL YLBL PROTEIN from Bacillus subtilis (350 aa), FASTA scores: opt: 385, E(): 5.6e-16, (27.7% identity in 350 aa overlap); etc. Protein product from Mb3216c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3216c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3D6" /db_xref="InterPro:IPR001478" /db_xref="InterPro:IPR008269" /db_xref="InterPro:IPR014721" /db_xref="InterPro:IPR020568" /db_xref="InterPro:IPR027065" /db_xref="InterPro:IPR036034" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3D6" BEGIN 1 MNRRILTLMV ALVPIVVFGV LLAVVTVPFV ALGPGPTFDT LGEIDGKQVV QIVGTQTYPT 61 SGHLNMTTVS QRDGLTLGEA LALWLSGQEQ LMPRDLVYPP GKSREEIEND NAADFKRSEA 121 AAEYAALGYL KYPKAVTVAS VMDPGPSVDK LQAGDAIDAV DGTPVGNLDQ FTALLKNTKP 181 GQEVTIDFRR KNEPPGIAQI TLGKNKDRDQ GVLGIEVVDA PWAPFAVDFH LANVGGPSAG 241 LMFSLAVVDK LTSGHLVGST FVAGTGTIAV DGKVGQIGGI THKMAAARAA GATVFLVPAK 301 NCYEASSDSP PGLKLVKVET LSQAVDALHA MTSGSPTPSC //