LOCUS SIU01823.1 299 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE NON-HEME HALOPEROXIDASE HPX protein. ACCESSION LT708304-3237 PROTEIN_ID SIU01823.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="hpx" /locus_tag="BQ2027_MB3196C" /note="Mb3196c, hpx, len: 299 aa. Equivalent to Rv3171c, len: 299 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 299 aa overlap). Possible hpx, non-heme haloperoxidase (EC 1.11.1.-), similar to other hydrolases (principaly epoxide hydrolases) and non-heme chloroperoxidases e.g. Q9RKB6|SCE87.22c PUTATIVE HYDROLASE from Streptomyces coelicolor (314 aa), FASTA scores: opt: 431, E(): 6e-20, (38.05% identity in 297 aa overlap); Q9HZ14|PA3226 PROBABLE HYDROLASE (SIMILAR TO ALPHA/BETA HYDROLASE FOLD) from Pseudomonas aeruginosa (275 aa), FASTA scores: opt: 236, E(): 1e-07, (29.6% identity in 277 aa overlap); Q9DBL9|1300003 D03RIK PROTEIN SIMILAR TO ALPHA/BETA HYDROLASE FOLD from Mus musculus (Mouse) (351 aa), FASTA scores: opt: 223, E(): 8.3e-07, (24.35% identity in 304 aa overlap); AAK46260|MT1988 EPOXIDE HYDROLASE from Mycobacterium tuberculosis strain CDC1551 (356 aa), FASTA scores: opt: 223, E(): 8.4e-07, (40.7% identity in 113 aa overlap); P49323|PRXC_STRLI|CPO|CPOL NON-HEME CHLOROPEROXIDASE (EC 1.11.1.10) (CHLORIDE PEROXIDASE) from Streptomyces lividans (275 aa), FASTA scores: opt: 220, E(): 1e-06, (29.5% identity in 305 aa overlap); etc. Equivalent to AAK47599 Hydrolase, alpha/beta hydrolase family from Mycobacterium tuberculosis strain CDC1551 but shorter 24 aa. Start chosen by similarity, alternative with good RBS possible. Protein product from Mb3196c detected using SWATH mass spectrometry. Mb3196c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3B7" /db_xref="InterPro:IPR022742" /db_xref="InterPro:IPR029058" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3B7" BEGIN 1 MTVRAADGTP LHTQVFGPPH GYPIVLTHGF VCAIRAWAYQ IADLAGDYRV IAFDHRGHGR 61 SGVPRRGAYS LNHLAADLDS VLDATLAPRE RAVVAGHSMG GITIAAWSDR YRHKVRRRTD 121 AVALINTTTG DLVRKVKLLS VPRELSPVRV LAGRSLVNTF GGFPLPGAAR ALSRHVISTL 181 AVAADADPSA TRLVYELFTQ MSAAGRGGCA KMLVEEVGSA HLNLDGLTVP TLVIGGVRDR 241 LTPISQSRRI ARTAPNVVGL VELPGGHCSM LERHQEVNSH LRALAESVTR HVRDRRISS //