LOCUS SIU01808.1 553 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE NADH DEHYDROGENASE I (CHAIN M) NUOK (NADH-UBIQUINONE OXIDOREDUCTASE CHAIN M) protein. ACCESSION LT708304-3222 PROTEIN_ID SIU01808.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="nuoM" /locus_tag="BQ2027_MB3181" /note="Mb3181, nuoM, len: 553 aa. Equivalent to Rv3157, len: 553 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 553 aa overlap). Probable nuoM, integral membrane NADH dehydrogenase I, chain M (EC 1.6.5.3), similar to others e.g. Q9XAR6|NUOM from Streptomyces coelicolor (523 aa), FASTA scores: opt: 1621, E(): 4.2e-89, (56.55% identity in 541 aa overlap); P50974|NUOM_RHOCA|NUOM from Rhodobacter capsulatus (Rhodopseudomonas capsulata) (512 aa), FASTA scores: opt: 996, E(): 6.5e-52, (38.2% identity in 521 aa overlap); P29925|NQOD_PARDE|NQO13 from Paracoccus denitrificans (513 aa), FASTA scores: opt: 987, E(): 2.2e-51, (37.05% identity in 540 aa overlap); etc. Also similar to MTCY251.04 (FASTA score: E(): 3.3e-16) and MTCY03A2.02c (FASTA score: E(): 9.6e-13). SIMILAR TO POLYPEPTIDE 4 OF THE NADH-UBIQUINOL OXIDOREDUCTASE OF CHLOROPLASTS OR MITOCHONDRIAL. Protein product from Mb3181 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3181 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3M4" /db_xref="InterPro:IPR001750" /db_xref="InterPro:IPR003918" /db_xref="InterPro:IPR010227" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3M4" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNNVPWLSVL WLVPLAGAVL IILLPPGRRR LAKWAGMVVS VLTLAVSIVV AAEFKPSAEP 61 YQFVEKHSWI PAFGAGYTLG VDGIAVVLVL LTTVLIPLLL VAGWNDATDA DDLSPASGRY 121 PQRPAPPRLR SSGGERTRGV HAYVALTLAI ESMVLMSVIA LDVLLFYVFF EAMLIPMYFL 181 IGGFGQGAGR SRAAVKFLLY NLFGGLIMLA AVIGLYVVTA QYDSGTFDFR EIVAGVAAGR 241 YGADPAVFKA LFLGFMFAFA IKAPLWPFHR WLPDAAVEST PATAVLMMAV MDKVGTFGML 301 RYCLQLFPDP STYFRPLIVT LAIIGVIYGA IVAIGQTDMM RLIAYTSISH FGFIIAGIFV 361 MTTQGQSGST LYMLNHGLST AAVFLIAGFL IARRDSRSIA DYGGVQKVAP ILAGTFMVSA 421 MATVSLPGLA PFISEFLVLL GTFSRYWLAA AFGVTALVLS AVYMLWLYQR VMTGPIAEGN 481 ERIGDLVGRE MIVVAPLIAL LLVLGVYPKP VLDIINPAVE NTMTTIGQHD PAPSVAHPVP 541 AVGASRTAEG PHP //