LOCUS       SIU01806.1                99 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            NADH DEHYDROGENASE I (CHAIN K) NUOK (NADH-UBIQUINONE OXIDOREDUCTASE 
            CHAIN K) protein.
ACCESSION   LT708304-3220
PROTEIN_ID  SIU01806.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="nuoK"
                     /locus_tag="BQ2027_MB3179"
                     /note="Mb3179, nuoK, len: 99 aa. Equivalent to Rv3155,
                     len: 99 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 99 aa overlap). Probable nuoK,
                     integral membrane NADH dehydrogenase I, chain K (EC
                     1.6.5.3), similar to others e.g. Q9XAR4|NUOK from
                     Streptomyces coelicolor (99 aa), FASTA scores: opt: 509,
                     E(): 2.7e-31, (78.55% identity in 98 aa overlap);
                     Q56226|NQOB_THETH|NQO11 from Thermus aquaticus (subsp.
                     thermophilus) (95 aa), BLAST scores: initn: 298, init1:
                     180, bits: 85.7, FASTA scores: opt: 313, E(): 9.4e-17,
                     (53.7% identity in 95 aa overlap); Q9RU97|DR1495 from
                     Deinococcus radiodurans (103 aa), FASTA scores: opt: 309,
                     E(): 2e-16, (52.0% identity in 100 aa overlap); etc. But
                     also similarity with NADH-PLASTOQUINONE OXIDOREDUCTASES
                     CHAIN 4L e.g. Q9MUL4|NULC_MESVI|NDHE from Mesostigma
                     viride (EC 1.6.5.3) (CATALYTIC ACTIVITY: NADH +
                     PLASTOQUINONE = NAD(+) + PLASTOQUINOL) (101 aa), FASTA
                     scores: opt: 280, E(): 2.8e-14, (40.6% identity in 101 aa
                     overlap); and P06261|NULC_TOBAC|NDHE|NDH4L from Nicotiana
                     tabacum (Common tobacco) (101 aa), FASTA scores: opt: 259,
                     E(): 1e-12, (43.0% identity in 93 aa overlap). SIMILAR TO
                     POLYPEPTIDE 4L OF THE NADH-UBIQUINOL OXIDOREDUCTASE OF
                     CHLOROPLASTS OR MITOCHONDRIA. Protein product from Mb3179
                     detected using shotgun mass spectrometry. Mb3179 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:P65566"
                     /db_xref="InterPro:IPR001133"
                     /db_xref="InterPro:IPR039428"
                     /db_xref="UniProtKB/Swiss-Prot:P65566"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MNPANYLYLS VLLFTIGASG VLLRRNAIVM FMCVELMLNA VNLAFVTFAR MHGHLDAQMI
       61 AFFTMVVAAC EVVVGLAIIM TIFRTRKSAS VDDANLLKG
//