LOCUS SIU01806.1 99 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE NADH DEHYDROGENASE I (CHAIN K) NUOK (NADH-UBIQUINONE OXIDOREDUCTASE CHAIN K) protein. ACCESSION LT708304-3220 PROTEIN_ID SIU01806.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="nuoK" /locus_tag="BQ2027_MB3179" /note="Mb3179, nuoK, len: 99 aa. Equivalent to Rv3155, len: 99 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 99 aa overlap). Probable nuoK, integral membrane NADH dehydrogenase I, chain K (EC 1.6.5.3), similar to others e.g. Q9XAR4|NUOK from Streptomyces coelicolor (99 aa), FASTA scores: opt: 509, E(): 2.7e-31, (78.55% identity in 98 aa overlap); Q56226|NQOB_THETH|NQO11 from Thermus aquaticus (subsp. thermophilus) (95 aa), BLAST scores: initn: 298, init1: 180, bits: 85.7, FASTA scores: opt: 313, E(): 9.4e-17, (53.7% identity in 95 aa overlap); Q9RU97|DR1495 from Deinococcus radiodurans (103 aa), FASTA scores: opt: 309, E(): 2e-16, (52.0% identity in 100 aa overlap); etc. But also similarity with NADH-PLASTOQUINONE OXIDOREDUCTASES CHAIN 4L e.g. Q9MUL4|NULC_MESVI|NDHE from Mesostigma viride (EC 1.6.5.3) (CATALYTIC ACTIVITY: NADH + PLASTOQUINONE = NAD(+) + PLASTOQUINOL) (101 aa), FASTA scores: opt: 280, E(): 2.8e-14, (40.6% identity in 101 aa overlap); and P06261|NULC_TOBAC|NDHE|NDH4L from Nicotiana tabacum (Common tobacco) (101 aa), FASTA scores: opt: 259, E(): 1e-12, (43.0% identity in 93 aa overlap). SIMILAR TO POLYPEPTIDE 4L OF THE NADH-UBIQUINOL OXIDOREDUCTASE OF CHLOROPLASTS OR MITOCHONDRIA. Protein product from Mb3179 detected using shotgun mass spectrometry. Mb3179 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65566" /db_xref="InterPro:IPR001133" /db_xref="InterPro:IPR039428" /db_xref="UniProtKB/Swiss-Prot:P65566" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNPANYLYLS VLLFTIGASG VLLRRNAIVM FMCVELMLNA VNLAFVTFAR MHGHLDAQMI 61 AFFTMVVAAC EVVVGLAIIM TIFRTRKSAS VDDANLLKG //