LOCUS SIU01804.1 211 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE NADH DEHYDROGENASE I (CHAIN I) NUOI (NADH-UBIQUINONE OXIDOREDUCTASE CHAIN I) protein. ACCESSION LT708304-3218 PROTEIN_ID SIU01804.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="nuoI" /locus_tag="BQ2027_MB3177" /note="Mb3177, nuoI, len: 211 aa. Equivalent to Rv3153, len: 211 aa, from Mycobacterium tuberculosis strain H37Rv, (99.5% identity in 211 aa overlap). Probable nuoI, NADH dehydrogenase I, chain I (EC 1.6.5.3), similar to others e.g. Q9XAR2|NUOI from Streptomyces coelicolor (211 aa), FASTA scores: opt: 825, E(): 9.3e-44, (70.1% identity in 164 aa overlap); Q56224|NQO9_THETH from Thermus aquaticus (subsp. thermophilus) (182 aa), FASTA scores: opt: 543, E(): 1.8e-26, (50.9% identity in 163 aa overlap); Q9RU95|DR1497 from Deinococcus radiodurans (178 aa), FASTA scores: opt: 527, E(): 1.7e-25, (48.75% identity in 162 aa overlap); etc. Contains two 4Fe-4S ferredoxins, iron-sulfur binding region signatures (PS00198). BELONGS TO THE COMPLEX I 23 KDA SUBUNIT FAMILY. THE IRON-SULFUR CENTERS ARE SIMILAR TO THOSE OF 'BACTERIAL-TYPE' 4FE-4S FERREDOXINS. COFACTOR: BINDS TWO 4FE-4S CLUSTERS. Protein product from Mb3177 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3177 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7TX57" /db_xref="InterPro:IPR010226" /db_xref="InterPro:IPR017896" /db_xref="InterPro:IPR017900" /db_xref="UniProtKB/Swiss-Prot:Q7TX57" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MANTDRPALP HKRAVPPSRA DSGPRRRRTK LLDAVAGFGV TLGSMFKKTV TEEYPERPGP 61 VAARYHGRHQ LNRYPDGLEK CIGCELCAWA CPADAIYVEG ADNTEEERFS PGERYGRVYQ 121 INYLRCIGCG LCIEACPTRA LTMTYDYELA DDNRADLIYE KDRLLAPLLP EMAAPPHPRA 181 PGATDKDYYL GNVTAEGLRG VRESQTTGDS R //