LOCUS SIU01801.1 445 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE NADH DEHYDROGENASE I (CHAIN F) NUOF (NADH-UBIQUINONE OXIDOREDUCTASE CHAIN F) protein. ACCESSION LT708304-3215 PROTEIN_ID SIU01801.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="nuoF" /locus_tag="BQ2027_MB3174" /note="Mb3174, nuoF, len: 445 aa. Equivalent to Rv3150, len: 445 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 445 aa overlap). Probable nuoF, NADH dehydrogenase, chain F (EC 1.6.5.3), similar to others e.g. Q9XAQ9|NUOF_STRCO from Streptomyces coelicolor (449 aa), FASTA scores: opt: 2314, E(): 3.5e-139, (76.25% identity in 434 aa overlap); NUF2_RHIME from Rhizobium meliloti (421 aa), FASTA scores: opt: 1545, E(): 1.8e-90, (53.1% identity in 424 aa overlap); Q9RU92|DR1500 from Deinococcus radiodurans (444 aa), FASTA scores: opt: 1445, E(): 4.1e-84, (52.9% identity in 427 aa overlap); etc. Contains respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2 (PS00645). BELONGS TO THE COMPLEX I 51 KDA SUBUNIT FAMILY. COFACTOR: FMN AND ONE 4FE-4S CLUSTER (PROBABLE). Protein product from Mb3174 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3174 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65568" /db_xref="InterPro:IPR001949" /db_xref="InterPro:IPR011537" /db_xref="InterPro:IPR011538" /db_xref="InterPro:IPR019554" /db_xref="InterPro:IPR019575" /db_xref="InterPro:IPR037207" /db_xref="InterPro:IPR037225" /db_xref="UniProtKB/Swiss-Prot:P65568" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTTQATPLTP VISRHWDDPE SWTLATYQRH DRYRGYQALQ KALTMPPDDV ISIVKDSGLR 61 GRGGAGFATG TKWSFIPQGD TGAAAKPHYL VVNADESEPG TCKDIPLMLA TPHVLIEGVI 121 IAAYAIRAHH AFVYVRGEVV PVLRRLHNAV AEAYAAGFLG RNIGGSGFDL ELVVHAGAGA 181 YICGEETALL DSLEGRRGQP RLRPPFPAVA GLYGCPTVIN NVETIASVPS IILGGIDWFR 241 SMGSEKSPGF TLYSLSGHVT RPGQYEAPLG ITLRELLDYA GGVRAGHRLK FWTPGGSSTP 301 LLTDEHLDVP LDYEGVGAAG SMLGTKALEI FDETTCVVRA VRRWTEFYKH ESCGKCTPCR 361 EGTFWLDKIY ERLETGRGSH EDIDKLLDIS DSILGKSFCA LGDGAASPVM SSIKHFRDEY 421 LAHVEGGGCP FDPRDSMLVA NGVDA //