LOCUS SIU01799.1 440 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE NADH DEHYDROGENASE I (CHAIN D) NUOD (NADH-UBIQUINONE OXIDOREDUCTASE CHAIN D) protein. ACCESSION LT708304-3213 PROTEIN_ID SIU01799.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="nuoD" /locus_tag="BQ2027_MB3172" /note="Mb3172, nuoD, len: 440 aa. Equivalent to Rv3148, len: 440 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 440 aa overlap). Probable nuoD, NADH dehydrogenase, chain B (EC 1.6.5.3), similar to others e.g. Q9XAQ7|NUOD from Streptomyces coelicolor (440 aa), FASTA scores: opt: 2198, E(): 1e-131, (73.9% identity in 429 aa overlap); P15689|NUCM_PARTE from Paramecium tetraurelia (400 aa), FASTA scores: opt: 922, E(): 5.8e-51, (38.5% identity in 408 aa overlap); Q9RU89|NUOD_DEIRA|DR1503 from Deinococcus radiodurans (401 aa), FASTA scores: opt: 922, E(): 5.8e-51, (47.75% identity in 404 aa overlap); etc. Equivalent to AAK47575 from Mycobacterium tuberculosis strain CDC1551 but longer 42 aa. Contains helix-turn-helix motif at aa 340-361. BELONGS TO THE COMPLEX I 49 KDA SUBUNIT FAMILY. Protein product from Mb3172 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3172 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65570" /db_xref="InterPro:IPR001135" /db_xref="InterPro:IPR014029" /db_xref="InterPro:IPR022885" /db_xref="InterPro:IPR029014" /db_xref="InterPro:IPR038290" /db_xref="UniProtKB/Swiss-Prot:P65570" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTAIADSAGG AGETVLVAGG QDWQQVVDAA RSADPGERIV VNMGPQHPST HGVLRLILEI 61 EGETVVEARC GIGYLHTGIE KNLEYRYWTQ GVTFVTRMDY LSPFFNETAY CLGVEKLLGI 121 TDEIPERVNV IRVLMMELNR ISSHLVALAT GGMELGAMTP MFVGFRAREI VLTLFEKITG 181 LRMNSAYIRP GGVAQDLPPN AATEIAEALK QLRQPLREMG ELLNENAIWK ARTQGVGYLD 241 LTGCMALGIT GPILRSTGLP HDLRKSEPYC GYQHYEFDVI TDDSCDAYGR YMIRVKEMWE 301 SMKIVEQCLD KLRPGPTMIS DRKLAWPADL QVGPDGLGNS PKHIAKIMGS SMEALIHHFK 361 LVTEGIRVPA GQVYVAVESP RGELGVHMVS DGGTRPYRVH YRDPSFTNLQ SVAAMCEGGM 421 VADLIAAVAS IDPVMGGVDR //