LOCUS SIU01797.1 184 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE NADH DEHYDROGENASE I (CHAIN B) NUOB (NADH-UBIQUINONE OXIDOREDUCTASE CHAIN B) protein. ACCESSION LT708304-3211 PROTEIN_ID SIU01797.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="nuoB" /locus_tag="BQ2027_MB3170" /note="Mb3170, nuoB, len: 184 aa. Equivalent to Rv3146, len: 184 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 184 aa overlap). Probable nuoB, NADH dehydrogenase, chain B (EC 1.6.5.3), similar to others e.g. Q9XAQ5|NUOB from Streptomyces coelicolor (184 aa), FASTA scores: opt: 989, E(): 1.4e-56, (78.25% identity in 184 aa overlap); Q56218|NQO6_THETH|NQO6 from Thermus aquaticus (subsp. thermophilus) (181 aa), FASTA scores: opt: 720, E(): 2.6e-39, (64.45% identity in 152 aa overlap); Q9RU87|DR1505 from Deinococcus radiodurans (181 aa), FASTA scores: opt: 719, E(): 3e-39, (62.6% identity in 155 aa overlap); etc. BELONGS TO THE COMPLEX I 20 KDA SUBUNIT FAMILY. MAY CONTAIN AN IRON-SULFUR 4FE-4S CLUSTER. TBparse score is 0.912. Protein product from Mb3170 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3170 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65576" /db_xref="InterPro:IPR006137" /db_xref="InterPro:IPR006138" /db_xref="UniProtKB/Swiss-Prot:P65576" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MGLEEQLPGG ILLSTVEKVA GYVRKNSLWP ATFGLACCAI EMMATAGPRF DIARFGMERF 61 SATPRQADLM IVAGRVSQKM APVLRQIYDQ MAEPKWVLAM GVCASSGGMF NNYAIVQGVD 121 HVVPVDIYLP GCPPRPEMLL HAILKLHEKI QQMPLGINRE RAIAEAEEAA LLARPTIEMR 181 GLLR //