LOCUS       SIU01789.1               362 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            PYRUVATE FORMATE LYASE ACTIVATING PROTEIN PFLA (FORMATE
            ACETYLTRANSFERASE ACTIVATING ENZYME) ([PYRUVATE FORMATE-
            LYASE] ACTIVATING ENZYME) protein.
ACCESSION   LT708304-3203
PROTEIN_ID  SIU01789.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="pflA"
                     /locus_tag="BQ2027_MB3162"
                     /note="Mb3162, pflA, len: 362 aa. Equivalent to Rv3138,
                     len: 362 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (98.9% identity in 362 aa overlap). Probable pflA,
                     pyruvate formate lyase activating protein (EC 1.97.1.4),
                     similar to other e.g. Q9V0N1|PAB1859 from Pyrococcus
                     abyssi (348 aa), FASTA scores: opt: 926, E(): 1.1e-52,
                     (39.95% identity in 343 aa overlap); O27446|MTH1395 from
                     Methanobacterium thermoautotrophicum (335 aa), FASTA
                     scores: opt: 909, E(): 1.3e-51, (42.2% identity in 327 aa
                     overlap); O28939|AF1330 from Archaeoglobus fulgidus (336
                     aa), FASTA scores: opt: 884, E(): 5.6e-50, (42.0% identity
                     in 319 aa overlap); etc. Also similar to O50099|PH1391
                     HYPOTHETICAL 40.2 KDA PROTEIN from Pyrococcus horikoshii
                     (348 aa), FASTA scores: opt: 934, E(): 3.3e-53, (40.5%
                     identity in 343 aa overlap); and other hypothetical
                     proteins. TBparse score is 0.881. Mb3162 found to be
                     expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y372"
                     /db_xref="InterPro:IPR007197"
                     /db_xref="InterPro:IPR013785"
                     /db_xref="InterPro:IPR016431"
                     /db_xref="InterPro:IPR027596"
                     /db_xref="InterPro:IPR034457"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y372"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MSDPFTIATK HWHRLHDSRI QCDVCPRACK LHEGQRGLCF VRGRFDDQVK LTSYGRSSGF
       61 CVDPIEKKPL NHFLPGSATL SFGTAGCNLA CKFCQNWDIS KSREIDVLAN RAAPADIART
      121 AHELGCRSVA FTYNDPTIFW EYAADVADAC HDQGIKAVAV TAGYMCPEPR AEFYRRVDAA
      181 NVDLKAFTED FYRKVCVSHL RNVLDTLAYL RHQTNVWLEI TTLLIPGRND SDAEVAAECR
      241 WIRENLGVDV PVHFTASHPD YKMMDTPATL PATLTRAREI GIGEGLRFVY TGNVHDAVGG
      301 STSCPGCRAT VIVRDWYSIR HYALTEDGRC QACGYQMPGV YDGPAGHWGQ RRLPLLTSLS
      361 RM
//