LOCUS SIU01789.1 362 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PYRUVATE FORMATE LYASE ACTIVATING PROTEIN PFLA (FORMATE ACETYLTRANSFERASE ACTIVATING ENZYME) ([PYRUVATE FORMATE- LYASE] ACTIVATING ENZYME) protein. ACCESSION LT708304-3203 PROTEIN_ID SIU01789.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pflA" /locus_tag="BQ2027_MB3162" /note="Mb3162, pflA, len: 362 aa. Equivalent to Rv3138, len: 362 aa, from Mycobacterium tuberculosis strain H37Rv, (98.9% identity in 362 aa overlap). Probable pflA, pyruvate formate lyase activating protein (EC 1.97.1.4), similar to other e.g. Q9V0N1|PAB1859 from Pyrococcus abyssi (348 aa), FASTA scores: opt: 926, E(): 1.1e-52, (39.95% identity in 343 aa overlap); O27446|MTH1395 from Methanobacterium thermoautotrophicum (335 aa), FASTA scores: opt: 909, E(): 1.3e-51, (42.2% identity in 327 aa overlap); O28939|AF1330 from Archaeoglobus fulgidus (336 aa), FASTA scores: opt: 884, E(): 5.6e-50, (42.0% identity in 319 aa overlap); etc. Also similar to O50099|PH1391 HYPOTHETICAL 40.2 KDA PROTEIN from Pyrococcus horikoshii (348 aa), FASTA scores: opt: 934, E(): 3.3e-53, (40.5% identity in 343 aa overlap); and other hypothetical proteins. TBparse score is 0.881. Mb3162 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y372" /db_xref="InterPro:IPR007197" /db_xref="InterPro:IPR013785" /db_xref="InterPro:IPR016431" /db_xref="InterPro:IPR027596" /db_xref="InterPro:IPR034457" /db_xref="UniProtKB/TrEMBL:A0A1R3Y372" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSDPFTIATK HWHRLHDSRI QCDVCPRACK LHEGQRGLCF VRGRFDDQVK LTSYGRSSGF 61 CVDPIEKKPL NHFLPGSATL SFGTAGCNLA CKFCQNWDIS KSREIDVLAN RAAPADIART 121 AHELGCRSVA FTYNDPTIFW EYAADVADAC HDQGIKAVAV TAGYMCPEPR AEFYRRVDAA 181 NVDLKAFTED FYRKVCVSHL RNVLDTLAYL RHQTNVWLEI TTLLIPGRND SDAEVAAECR 241 WIRENLGVDV PVHFTASHPD YKMMDTPATL PATLTRAREI GIGEGLRFVY TGNVHDAVGG 301 STSCPGCRAT VIVRDWYSIR HYALTEDGRC QACGYQMPGV YDGPAGHWGQ RRLPLLTSLS 361 RM //