LOCUS SIU01783.1 217 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 TWO COMPONENT TRANSCRIPTIONAL REGULATORY PROTEIN DEVR (PROBABLY LUXR/UHPA-FAMILY) protein. ACCESSION LT708304-3197 PROTEIN_ID SIU01783.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="devR" /locus_tag="BQ2027_MB3157C" /note="Mb3157c, devR, len: 217 aa. Equivalent to Rv3133c, len: 217 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 217 aa overlap). devR, two component transcriptional regulator (see first citation below; dev for Differentially Expressed in Virulent strain), highly similar to several e.g. O85372|CPRR TWO COMPONENT REGULATOR from Rhodococcus sp. (212 aa), FASTA scores: opt: 868, E(): 6.2e-46, (65.05% identity in 206 aa overlap); Q9RI42|SCJ12.16c PUTATIVE LUXR FAMILY TWO-COMPONENT RESPONSE REGULATOR from Streptomyces coelicolor (233 aa), FASTA scores: opt: 849, E(): 9.7e-45, (60.55% identity in 218 aa overlap); Q9XA59|SCGD3.19 PUTATIVE TWO-COMPONENT SYSTEM RESPONSE TRANSCRIPTIONAL REGULATOR from Streptomyces coelicolor (218 aa), FASTA scores: opt: 835, E(): 6.5e-44, (61.55% identity in 208 aa overlap); and similar to others. Contains bacterial regulatory proteins, LuxR family signature (PS00622) near C-terminus as seen in bvgA, comA, dctR, degU, evgA, fimZ, fixJ, gacA, glpR, narL, narP, nodW, rcsB and uhpA. Helix-turn-helix motif at 166-187 (+3.15 SD). BELONGS TO THE LUXR/UHPA FAMILY OF TRANSCRIPTIONAL REGULATORS. THE N-TERMINAL REGION IS SIMILAR TO THAT OF OTHER REGULATORY COMPONENTS OF SENSORY TRANSDUCTION SYSTEMS. Protein product from Mb3157c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3157c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y377" /db_xref="InterPro:IPR000792" /db_xref="InterPro:IPR001789" /db_xref="InterPro:IPR011006" /db_xref="InterPro:IPR016032" /db_xref="UniProtKB/TrEMBL:A0A1R3Y377" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MVKVFLVDDH EVVRRGLVDL LGADPELDVV GEAGSVAEAM ARVPAARPDV AVLDVRLPDG 61 NGIELCRDLL SRMPDLRCLI LTSYTSDEAM LDAILAGASG YVVKDIKGME LARAVKDVGA 121 GRSLLDNRAA AALMAKLRGA AEKQDPLSGL TDQERTLLGL LSEGLTNKQI ADRMFLAEKT 181 VKNYVSRLLA KLGMERRTQA AVFATELKRS RPPGDGP //