LOCUS SIU01782.1 578 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 TWO COMPONENT SENSOR HISTIDINE KINASE DEVS protein. ACCESSION LT708304-3196 PROTEIN_ID SIU01782.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="devS" /locus_tag="BQ2027_MB3156C" /note="Mb3156c, devS, len: 578 aa. Equivalent to Rv3132c, len: 578 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 578 aa overlap). devS, membrane-bound two component sensor histidine kinase (EC 2.7.3.-) (see citations below; dev for Differentially Expressed in Virulent strain), similar to others two component sensors e.g. Q9RI43|SCJ12.15c PUTATIVE TWO-COMPONENT SENSOR from Streptomyces coelicolor (585 aa), FASTA scores: opt: 1305, E(): 2.5e-69, (41.35% identity in 573 aa overlap); Q9ZBY4|SCD78.15 PUTATIVE TWO COMPONENT SENSOR from Streptomyces coelicolor (560 aa), FASTA scores: opt: 1194, E(): 8.1e-63, (41.05% identity in 558 aa overlap); O85371|CPRS TWO COMPONENT REGULATOR from Rhodococcus sp (563 aa), FASTA scores: opt: 803, E(): 8.3e-40, (38.4% identity in 552 aa overlap); Q9L094|SCC24.23 PUTATIVE TWO-COMPONENT SENSOR HISTIDINE KINASE from Streptomyces coelicolor (similarity only in C-terminus for this one); etc. Also highly similar to mycobacterium O53473|Rv2027c|MTV018.14c PUTATIVE MEMBRANE PROTEIN (573 aa), FASTA scores: opt: 2333, E(): 7.6e-130, (61.45% identity in 576 aa overlap). Protein product from Mb3156c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3156c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y364" /db_xref="InterPro:IPR003018" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR005467" /db_xref="InterPro:IPR011712" /db_xref="InterPro:IPR029016" /db_xref="InterPro:IPR036890" /db_xref="UniProtKB/TrEMBL:A0A1R3Y364" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTTGGLVDEN DGAAMRPLRH TLSQLRLHEL LVEVQDRVEQ IVEGRDRLDG LVEAMLVVTA 61 GLDLEATLRA IVHSATSLVD ARYGAMEVHD RQHRVLHFVY EGIDEETVRR IGHLPKGLGV 121 IGLLIEDPKP LRLDDVSAHP ASIGFPPYHP PMRTFLGVPV RVRDESFGTL YLTDKTNGQP 181 FSDDDEVLVQ ALAAAAGIAV ANARLYQQAK ARQSWIEATR DIATELLSGT EPATVFRLVA 241 AEALKLTAAD AALVAVPVDE DMPAADVGEL LVIETVGSAV ASTVGRTIPV AGAVLREVFV 301 NGIPRRVDRV DLEGLDELAD AGPALLLPLR ARGTVAGVVV VLSQGGPGAF TDEQLEMMAA 361 FADQAALAWQ LATSQRRMRE LDVLTDRDRI ARDLHDHVIQ RLFAIGLALQ GAVPHERNPE 421 VQQRLSDVVD DLQDVIQEIR TTIYDLHGAS QGITRLRQRI DAAVAQFADS GLRTSVQFVG 481 PLSVVDSALA DQAEAVVREA VSNAVRHAKA STLTVRVKVD DDLCIEVTDN GRGLPDEFTG 541 SGLTNLRQRA EQAGGEFTLA SVPGASGTVL RWSAPLSQ //