LOCUS SIU01738.1 368 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ZINC-TYPE ALCOHOL DEHYDROGENASE ADHD (ALDEHYDE REDUCTASE) protein. ACCESSION LT708304-3152 PROTEIN_ID SIU01738.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="adhD" /locus_tag="BQ2027_MB3113" /note="Mb3113, adhD, len: 368 aa. Equivalent to Rv3086, len: 368 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 368 aa overlap). Probable adhD, zinc-type alcohol dehydrogenase (EC 1.1.1.-), highly similar to many e.g. O69045 HYPOTHETICAL ALCOHOL DEHYDROGENASE from Rhodococcus rhodochrous (370 aa), FASTA scores: opt: 1255, E(): 8.7e-68, (50.4% identity in 367 aa overlap); P25406|ADHB_UROHA ALCOHOL DEHYDROGENASE I-B from Uromastyx hardwickii (Indian spiny-tailed lizard) (375 aa), FASTA scores: opt: 787, E(): 8.2e-40, (35.9% identity in 373 aa overlap); P72324||ADHI_RHOSH ALCOHOL DEHYDROGENASE CLASS III from Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) (376 aa), FASTA scores: opt: 787, E(): 8.3e-40, (35.1% identity in 379 aa overlap). Also highly similar to P71818|Rv0761c|MTCY369.06c HYPOTHETICAL ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN from Mycobacterium tuberculosis strain H37Rv (375 aa), FASTA scores: opt: 1186, E(): 1.2e-63, (47.3% identity in 368 aa overlap). Contains PS00059 Zinc-containing alcohol dehydrogenases signature. BELONGS TO THE ZINC-CONTAINING ALCOHOL DEHYDROGENASE. POSSIBLY REQUIRES ZINC FOR ITS ACTIVITY. Protein product from Mb3113 detected using SWATH mass spectrometry. Mb3113 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y3F2" /db_xref="InterPro:IPR002328" /db_xref="InterPro:IPR011032" /db_xref="InterPro:IPR013149" /db_xref="InterPro:IPR013154" /db_xref="InterPro:IPR020843" /db_xref="InterPro:IPR023921" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3F2" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MKTTAAVLFE AGKPFELMEL DLDGPGPGEV LVKYTAAGLC HSDLHLTDGD LPPRFPIVGG 61 HEGSGVIEEV GAGVTRVKPG DHVVCSFIPN CGTCRYCCTG RQNLCDMGAT ILEGCMPDGS 121 FRFHSQGTDF GAMCMLGTFA ERATVSQHSV VKVDDWLPLE TAVLVGCGVP SGWGTAVNAG 181 NLRAGDTAVI YGVGGLGINA VQGATAAGCK YVVVVDPVAF KRETALKFGA THAFADAASA 241 AAKVDELTWG QGADAALILV GTVDDEVVSA ATAVIGKGGT VVITGLADPA KLTVHVSGTD 301 LTLHEKTIKG SLFGSCNPQY DIVRLLRLYD AGQLMLDELV TTTYNLEQVN QGYQDLRDGK 361 NIRGVIVH //