LOCUS SIU01714.1 507 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable
atp-dependent dna ligase ligb (polydeoxyribonucleotide
synthase [atp]) (polynucleotide ligase [atp]) (sealase)
(dna repair protein) (dna joinase) protein.
ACCESSION LT708304-3128
PROTEIN_ID SIU01714.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="ligB"
/locus_tag="BQ2027_MB3089"
/note="Mb3089, ligB, len: 507 aa. Equivalent to Rv3062,
len: 507 aa, from Mycobacterium tuberculosis strain H37Rv,
(99.8% identity in 507 aa overlap). Probable ligB, DNA
ligase ATP-dependent (EC 6.5.1.1), highly similar to
numerous archaebacterial and eukaryotic polynucleotide DNA
ligases, e.g. Q9FCB1|DNLI_STRCO|LIG|2SCG58.02 from
Streptomyces coelicolor (512 aa), FASTA scores: opt: 1677,
E(): 2.5e-90, (55.65% identity in 512 aa overlap);
Q9HR35|DNLI_HALN1|LIG|VNG0881G from Halobacterium sp.
strain NRC-1 (561 aa), FASTA scores: opt: 985, E():
5.6e-50, (42.25% identity in 440 aa overlap);
Q9V185|DNLI_PYRAB|LIG|PAB2002 from Pyrococcus abyssi (559
aa), FASTA scores: opt: 978, E(): 1.4e-49, (39.05%
identity in 443 aa overlap); etc. Also similar to
Rv3731|MTV025.079|LIGC POSSIBLE DNA LIGASE from M.
tuberculosis (358 aa). Similarity at N-terminus is poor so
first start codon was taken. Contains (PS00697)
ATP-dependent DNA ligase AMP-binding site signature, and
(PS00017) ATP/GTP-binding site motif A (P-loop). BELONGS
TO THE ATP-DEPENDENT DNA LIGASE FAMILY. Protein product
from Mb3089 detected using SWATH mass spectrometry. Mb3089
found to be expressed during exponential growth in
Sauton's minimal media by RNA-sequencing."
/db_xref="GOA:Q7TTR7"
/db_xref="InterPro:IPR000977"
/db_xref="InterPro:IPR012308"
/db_xref="InterPro:IPR012309"
/db_xref="InterPro:IPR012310"
/db_xref="InterPro:IPR012340"
/db_xref="InterPro:IPR016059"
/db_xref="InterPro:IPR022865"
/db_xref="InterPro:IPR036599"
/db_xref="UniProtKB/Swiss-Prot:Q7TTR7"
BEGIN
1 MLLHDVAITS MDVAATSSRL TKVARIAALL HRAAPDTQLV TIIVSWLSGE LPQRHIGVGW
61 AALRSLPPPA PQPALTVTGV DATLSKIGTL SGKGSQAQRA ALVAELFSAA TEAEQTFLLR
121 LLGGELRQGA KGGIMADAVA QAAGLPAATV QRAAMLGGDL AAAAAAGLSG AALDTFTLRV
181 GRPIGPMLAQ TATSVHDALE RHGGTTIFEA KLDGARVQIH RANDQVRIYT RSLDDVTARL
241 PEVVEATLAL PVRDLVADGE AIALCPDNRP QRFQVTASRF GRSVDVAAAR ATQPLSVFFF
301 DILHRDGTDL LEAPTTERLA ALDALVPARH RVDRLITSDP TDAANFLDAT LAAGHEGVMA
361 KAPAARYLAG RRGAGWLKVK PVHTLDLVVL AVEWGSGRRR GKLSNIHLGA RDPATGGFVM
421 VGKTFKGMTD AMLDWQTTRF HEIAVGPTDG YVVQLRPEQV VEVALDGVQR SSRYPGGLAL
481 RFARVVRYRA DKDPAEADTI DAVRALY
//