LOCUS SIU01712.1 600 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ACYL-COA DEHYDROGENASE FADE22b [SECOND PART] protein. ACCESSION LT708304-3126 PROTEIN_ID SIU01712.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadE22b" /locus_tag="BQ2027_MB3087C" /note="Mb3087c, fadE22b, len: 600 aa. Equivalent to 3' end of Rv3061c, len: 721 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 600 aa overlap). Probable fadE22, Acyl-CoA Dehydrogenase (EC 1.3.99.-), similar to many e.g. AAK44503|MT0284 from Mycobacterium tuberculosis strain CDC1551 (731 aa), FASTA scores: opt: 1804, E(): 1.1e-101, (43.45% identity in 743 aa overlap); AAK48037|MT3678 from Mycobacterium tuberculosis strain CDC1551 (711 aa), FASTA scores: opt: 1630, E(): 3.9e-91, (42.55% identity in 733 aa overlap); and extensive similarity in C-terminal part to many acyl-CoA dehydrogenases e.g. Q9A5G9|CC2478 from Caulobacter crescentus (407 aa), FASTA scores: opt: 767, E(): 4.8e-39, (36.7% identity in 376 aa overlap). Also similar to many hypothetical proteins. COULD BELONG TO THE ACYL-COA DEHYDROGENASES FAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, fadE22 exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base insertion (*-c) splits fadE22 into 2 parts, fadE22a and fadE22b. Protein product from Mb3087c detected using SWATH mass spectrometry. Mb3087c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y2Z8" /db_xref="InterPro:IPR006091" /db_xref="InterPro:IPR009075" /db_xref="InterPro:IPR009100" /db_xref="InterPro:IPR013786" /db_xref="InterPro:IPR036250" /db_xref="InterPro:IPR037069" /db_xref="UniProtKB/TrEMBL:A0A1R3Y2Z8" BEGIN 1 MGLDSQVQVT DGVADGEAGI VLGAGLAELL LVAAGDDVLV LERGRKGVSV DVPENFDPTR 61 RSGRVRLDNV RVTTDDILLG AYESALARAR TLLAAEAVGG AADCVDSAVA YAKVRQQFGR 121 TIATFQAVKH HCANMLVAAE SAIAAVWDAA RAAAEDEEQF RLAAAVAAAL AFPAYARNAE 181 LNIQVHGGIG FTWEHDAHLH LRRALVTVGL FGGDAPVRDV FERTAAGVTR AISLDLPAQA 241 EELRARIRSD AAEIAALEKD AQRDKLIETG YVMPHWPRPW GRAAGAVEQL VIEEEFSAAG 301 IERPDYSITG WVILTLIQHG TPWQIERFVE KALRQQEIWC QLFSEPDAGS DAASVKTRAT 361 RVEGGWKING QKVWTSGAQY CARGLATVRT DPDAPKHAGI TTVIIDMLAP GVEVRPLRQI 421 TGDSEFNEVF FNDVFVPDED VVGAPNSGWT VARATLGNER VSIGGSGSYY EAMAAKLVQL 481 VQRRSDAFAG APIRVGAFLA EDHALRLLNL RRAARSVEGA GPGPEGNITK LKVAEHMIEG 541 AAIAAALWGP EIALLDGPGR VIGRTVMGAR GMAIAGGTSE VTRNQIAERI LGMPRDPLIS //