LOCUS SIU01710.1 492 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CYTOCHROME P450 136 CYP136 protein. ACCESSION LT708304-3124 PROTEIN_ID SIU01710.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cyp136" /locus_tag="BQ2027_MB3085" /note="Mb3085, cyp136, len: 492 aa. Equivalent to Rv3059, len: 492 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 492 aa overlap). Probable cyp136, cytochrome P450 136 (EC 1.14.-.-), similar to other cytochrome P450-dependent oxidases e.g. Q59990|CYP120|CYP|SLR0574 PUTATIVE CYTOCHROME P450 120 from Synechocystis sp. strain PCC 6803 (444 aa), FASTA scores: opt: 579, E(): 1.5e-29, (27.3% identity in 443 aa overlap); Q64654|CYP51|CP51_RAT CYTOCHROME P450 51 (EC 1.14.14.-) (LANOSTEROL 14-ALPHA DEMETHYLASE) from Rattus norvegicus (Rat) (503 aa), FASTA scores: opt: 549, E(): 1.4e-27, (26.2% identity in 458 aa overlap); Q9JIY3|CYP51 LANOSTEROL 14-ALPHA-DEMETHYLASE from Mus musculus (Mouse) (486 aa), FASTA scores: opt: 546, E(): 2.1e-27, (25.75% identity in 458 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). BELONGS TO THE CYTOCHROME P450 FAMILY. Protein product from Mb3085 detected using SWATH mass spectrometry. Mb3085 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3Y314" /db_xref="InterPro:IPR001128" /db_xref="InterPro:IPR002403" /db_xref="InterPro:IPR017972" /db_xref="InterPro:IPR036396" /db_xref="UniProtKB/TrEMBL:A0A1R3Y314" BEGIN 1 MATIHPPAYL LDQAKRRFTP SFNNFPGMSL VEHMLLNTKF PEKKLAEPPP GSGLKPVVGD 61 AGLPILGHMI EMLRGGPDYL MFLYKTKGPV VFGDSAVLPG VAALGPDAAQ VIYSNRNKDY 121 SQQGWVPVIG PFFHRGLMLL DFEEHMFHRR IMQEAFVRSR LAGYLEQMDR VVSRVVADDW 181 VVNDARFLVY PAMKALTLDI ASMVFMGHEP GTDHELVTKV NKAFTITTRA GNAVIRTSVP 241 PFTWWRGLRA RELLENYFTA RVKERREASG NDLLTVLCQT EDDDGNRFSD ADIVNHMIFL 301 MMAAHDTSTS TATTMAYQLA AHPEWQQRCR DESDRHGDGP LDIESLEQLE SLDLVMNESI 361 RLVTPVQWAM RQTVRDTELL GYYLPKGTNV IAYPGMNHRL PEIWTDPLTF DPERFTEPRN 421 EHKRHRYAFT PFGGGVHKCI GMVFGQLEIK TILHRLLRRY RLELSRPDYQ PRWDYSAMPI 481 PMDGMPIVLR PR //