LOCUS SIU01687.1 227 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED SECRETED PROTEIN TB22.2 protein. ACCESSION LT708304-3101 PROTEIN_ID SIU01687.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="TB22.2" /locus_tag="BQ2027_MB3062C" /note="Mb3062c, TB22.2, len: 227 aa. Equivalent to Rv3036c, len: 227 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 227 aa overlap). Probable TB22.2, conserved secreted protein, with putative N-terminal signal peptide, highly similar to secreted immunogenic protein MPT64/MPB64 P19996|Rv1980c|MTCY39.39 from Mycobacterium tuberculosis and Mycobacterium bovis (228 aa), FASTA scores: opt: 681, E(): 2.5e-35, (45.8% identity in 227 aa overlap). Protein product from Mb3062c detected using shotgun mass spectrometry. Mb3062c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="InterPro:IPR021729" /db_xref="InterPro:IPR037126" /db_xref="UniProtKB/TrEMBL:A0A1R3Y336" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MRYLIATAVL VAVVLVGWPA AGAPPSCAGL GGTVQAGQIC HVHASGPKYM LDMTFPVDYP 61 DQRALTDYIT QNRDGFVNVA QGSPLRDQPY QMDATSEQHS SGQPPQATRS VVLKFFQDLG 121 GAHPSTWYKA FNYNLATSQP ITFDTLFVPG TTPLDSIYPI VQRELARQTG FGAAILPSTG 181 LDPAHYQNFA ITDDSLIFYF AQGELLPSFV GACQAQVPRS AIPPLAI //