LOCUS       SIU01678.1               318 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ELECTRON TRANSFER FLAVOPROTEIN (ALPHA-SUBUNIT) FIXB (ALPHA-
            ETF) (ELECTRON TRANSFER FLAVOPROTEIN LARGE SUBUNIT) (ETFLS)
            protein.
ACCESSION   LT708304-3092
PROTEIN_ID  SIU01678.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fixB"
                     /locus_tag="BQ2027_MB3054C"
                     /standard_name="etfA"
                     /note="Mb3054c, fixB, len: 318 aa. Equivalent to Rv3028c,
                     len: 318 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.7% identity in 318 aa overlap). Probable fixB
                     (alternate gene name: etfA), electron transfer
                     flavoprotein (alpha subunit) for various dehydrogenases.
                     Equivalent to O33096|ETFA_MYCLE|FIXB|ML1711|MLCB637.04
                     ELECTRON TRANSFER FLAVOPROTEIN from Mycobacterium leprae
                     (318 aa), FASTA scores: opt: 1788, E(): 1.1e-87, (89.3%
                     identity in 318 aa overlap). Also highly similar to many
                     e.g. Q9K418|SCG22.27c from Streptomyces coelicolor (320
                     aa), FASTA scores: opt: 1161, E(): 1.6e-54, (59.45%
                     identity in 323 aa overlap); AAK08137|etfa from
                     Rhodobacter sphaeroides (308 aa), FASTA scores: opt: 792,
                     E(): 5.1e-35, (45.95% identity in 309 aa overlap);
                     P38974|ETFA_PARDE ELECTRON TRANSFER FLAVOPROTEIN from
                     Paracoccus denitrificans (307 aa), FASTA scores: opt: 789,
                     E(): 7.4e-35, (45.95% identity in 309 aa overlap); etc.
                     BELONGS TO THE ETF ALPHA-SUBUNIT / FIXB FAMILY. Protein
                     product from Mb3054c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb3054c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y3A9"
                     /db_xref="InterPro:IPR001308"
                     /db_xref="InterPro:IPR014729"
                     /db_xref="InterPro:IPR014730"
                     /db_xref="InterPro:IPR014731"
                     /db_xref="InterPro:IPR018206"
                     /db_xref="InterPro:IPR029035"
                     /db_xref="InterPro:IPR033947"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y3A9"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MAEVLVLVEH AEGALKKVSA ELITAARALG EPAAVVVGVP GTAAPLVDGL KAAGAAKIYV
       61 AESDLVDKYL ITPAVDVLAG LAESSAPAGV LIAATADGKE IAGRLAARIG SGLLVDVVDV
      121 REGGVGVHSI FGGAFTVEAQ ANGDTPVITV RAGAVEAEPA AGAGEQVSVE VPAAAENAAR
      181 ITAREPAVAG DRPELTEATI VVAGGRGVGS AENFSVVEAL ADSLGAAVGA SRAAVDSGYY
      241 PGQFQVGQTG KTVSPQLYIA LGISGAIQHR AGMQTSKTIV AVNKDEEAPI FEIADYGVVG
      301 DLFKVAPQLT EVIKARKG
//