LOCUS SIU01678.1 318 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ELECTRON TRANSFER FLAVOPROTEIN (ALPHA-SUBUNIT) FIXB (ALPHA- ETF) (ELECTRON TRANSFER FLAVOPROTEIN LARGE SUBUNIT) (ETFLS) protein. ACCESSION LT708304-3092 PROTEIN_ID SIU01678.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fixB" /locus_tag="BQ2027_MB3054C" /standard_name="etfA" /note="Mb3054c, fixB, len: 318 aa. Equivalent to Rv3028c, len: 318 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 318 aa overlap). Probable fixB (alternate gene name: etfA), electron transfer flavoprotein (alpha subunit) for various dehydrogenases. Equivalent to O33096|ETFA_MYCLE|FIXB|ML1711|MLCB637.04 ELECTRON TRANSFER FLAVOPROTEIN from Mycobacterium leprae (318 aa), FASTA scores: opt: 1788, E(): 1.1e-87, (89.3% identity in 318 aa overlap). Also highly similar to many e.g. Q9K418|SCG22.27c from Streptomyces coelicolor (320 aa), FASTA scores: opt: 1161, E(): 1.6e-54, (59.45% identity in 323 aa overlap); AAK08137|etfa from Rhodobacter sphaeroides (308 aa), FASTA scores: opt: 792, E(): 5.1e-35, (45.95% identity in 309 aa overlap); P38974|ETFA_PARDE ELECTRON TRANSFER FLAVOPROTEIN from Paracoccus denitrificans (307 aa), FASTA scores: opt: 789, E(): 7.4e-35, (45.95% identity in 309 aa overlap); etc. BELONGS TO THE ETF ALPHA-SUBUNIT / FIXB FAMILY. Protein product from Mb3054c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb3054c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3Y3A9" /db_xref="InterPro:IPR001308" /db_xref="InterPro:IPR014729" /db_xref="InterPro:IPR014730" /db_xref="InterPro:IPR014731" /db_xref="InterPro:IPR018206" /db_xref="InterPro:IPR029035" /db_xref="InterPro:IPR033947" /db_xref="UniProtKB/TrEMBL:A0A1R3Y3A9" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MAEVLVLVEH AEGALKKVSA ELITAARALG EPAAVVVGVP GTAAPLVDGL KAAGAAKIYV 61 AESDLVDKYL ITPAVDVLAG LAESSAPAGV LIAATADGKE IAGRLAARIG SGLLVDVVDV 121 REGGVGVHSI FGGAFTVEAQ ANGDTPVITV RAGAVEAEPA AGAGEQVSVE VPAAAENAAR 181 ITAREPAVAG DRPELTEATI VVAGGRGVGS AENFSVVEAL ADSLGAAVGA SRAAVDSGYY 241 PGQFQVGQTG KTVSPQLYIA LGISGAIQHR AGMQTSKTIV AVNKDEEAPI FEIADYGVVG 301 DLFKVAPQLT EVIKARKG //