LOCUS       SIU01675.1               393 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 cysteine
            desulfurase iscs (nifs protein homolog) (nitrogenase metalloclusters
            biosynthesis protein nifs) protein.
ACCESSION   LT708304-3089
PROTEIN_ID  SIU01675.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="iscS"
                     /locus_tag="BQ2027_MB3051C"
                     /standard_name="nifS"
                     /note="Mb3051c, iscS, len: 393 aa. Equivalent to Rv3025c,
                     len: 393 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 393 aa overlap). Probable iscS
                     (alternate gene name: nifS), cysteine desulfurase
                     (NifS-like protein) (EC 4.4.1.-), equivalent to
                     MLCB637.06|O33098 NIFS-LIKE PROTEIN from Mycobacterium
                     leprae (396 aa), FASTA scores: opt: 2186, E(): 2.7e-122,
                     (84.9% identity in 391 aa overlap). Also highly similar to
                     many e.g. O86581|SC2A11.20 PUTATIVE
                     PYRIDOXAL-PHOSPHATE-DEPENDENT AMINOTRANSFERASE from
                     Streptomyces coelicolor (389 aa), FASTA scores: opt: 1568,
                     E(): 1.1e-85, (61.7% identity in 389 aa overlap);
                     P57795|ISCS|NIFS CYSTEINE DESULFURASE (NIFS PROTEIN
                     HOMOLOG) from Methanosarcina thermophila (404 aa), FASTA
                     scores: opt: 1059, E(): 1.6e-55, (46.2% identity in 381 aa
                     overlap); O54055|ISCS_RUMFL|ISCS|NIFS CYSTEINE DESULFURASE
                     from Ruminococcus flavefaciens (396 aa), FASTA scores:
                     opt: 973, E(): 2e-50, (43.3% identity in 381 aa overlap);
                     P57794|NIFS_ACEDI CYSTEINE DESULFURASE from Acetobacter
                     diazotrophicus (400 aa), FASTA scores: opt: 958, E():
                     1.6e-49, (41.1% identity in 392 aa overlap); etc. Also
                     similar to Rv1464|MTV007.11 from Mycobacterium
                     tuberculosis. Contains PS00595 Aminotransferases class-V
                     pyridoxal-phosphate attachment site. BELONGS TO CLASS-V OF
                     PYRIDOXAL-PHOSPHATE-DEPENDENT AMINOTRANSFERASES, NIFS/ISCS
                     SUBFAMILY. COFACTOR: PYRIDOXAL PHOSPHATE (BY SIMILARITY).
                     Protein product from Mb3051c detected using SWATH mass
                     spectrometry. Mb3051c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3Y4T3"
                     /db_xref="InterPro:IPR000192"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015422"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="InterPro:IPR016454"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y4T3"
BEGIN
        1 MAYLDHAATT PMHPAAIEAM AAVQRTIGNA SSLHTSGRSA RRRIEEAREL IADKLGARPS
       61 EVIFTAGGTE SDNLAVKGIY WARRDAEPHR RRIVTTEVEH HAVLDSVNWL VEHEGAHVTW
      121 LPTAADGSVS ATALREALQS HDDVALVSVM WANNEVGTIL PIAEMSVVAM EFGVPMHSDA
      181 IQAVGQLPLD FGASGLSAMS VAGHKFGGPP GVGALLLRRD VTCVPLMHGG GQERDIRSGT
      241 PDVASAVGMA TAAQIAVDGL EENSARLRLL RDRLVEGVLA EIDDVCLNGA DDPMRLAGNA
      301 HFTFRGCEGD ALLMLLDANG IECSTGSACT AGVAQPSHVL IAMGVDAASA RGSLRLSLGH
      361 TSVEADVDAA LEVLPGAVAR ARRAALAAAG ASR
//